Le Bivic A, Arsanto J P
Laboratoire de Biologie de la Différenciation cellulaire, U.A. 179, Faculté des Sciences de Luminy, Marseille, France.
Biol Cell. 1987;60(1):41-7. doi: 10.1111/j.1768-322x.1987.tb00544.x.
The expression and cytochemical localization of alkaline phosphatase and Na+-pump sites were investigated in the human adenocarcinoma cell line HT-29.18 during differentiation. In the undifferentiated state, HT-29.18 cells expressed ATPase activity on plasma membrane whereas they displayed no alkaline phosphatase activity. In differentiated HT-29.18 cells, strong alkaline phosphatase activity was present on the apical membrane, whereas ATPase activity was restricted to the basolateral membrane. Intra- and intercellular lumina (cysts) observed in undifferentiated cells were devoid of both enzyme activities. In differentiated cells, cysts bearing well developed microvilli were strongly positive for alkaline phosphatase activity, while this activity seemed to be lacking in cysts without microvilli. ATPase activity was not found in either type of structure. Finally, HT-29.18 differentiated cells expressed, at pH 9.0, a p-nitrophenylphosphatase activity six-fold greater than that of undifferentiated cells.
在人腺癌细胞系HT - 29.18分化过程中，对碱性磷酸酶和钠泵位点的表达及细胞化学定位进行了研究。在未分化状态下，HT - 29.18细胞在质膜上表达ATP酶活性，而未显示碱性磷酸酶活性。在分化的HT - 29.18细胞中，顶端膜上存在强碱性磷酸酶活性，而ATP酶活性局限于基底外侧膜。在未分化细胞中观察到的细胞内和细胞间腔隙（囊肿）均缺乏这两种酶活性。在分化细胞中，带有发育良好微绒毛的囊肿碱性磷酸酶活性呈强阳性，而无微绒毛的囊肿似乎缺乏这种活性。两种结构中均未发现ATP酶活性。最后，HT - 29.18分化细胞在pH 9.0时表达的对硝基苯磷酸酶活性比未分化细胞高六倍。
