Institute of Molecular and Translational Medicine Dentistry, Faculty of Medicine and Dentistry and Czech Advanced Technology Research Institute, Palacký University in Olomouc, Hněvotínská 5, 779 00 Olomouc, Czech Republic.
Molecules. 2021 Sep 11;26(18):5515. doi: 10.3390/molecules26185515.
Cell quantification is widely used both in basic and applied research. A typical example of its use is drug discovery research. Presently, plenty of methods for cell quantification are available. In this review, the basic techniques used for cell quantification, with a special emphasis on techniques based on fluorescent DNA dyes, are described. The main aim of this review is to guide readers through the possibilities of cell quantification with various methods and to show the strengths and weaknesses of these methods, especially with respect to their sensitivity, accuracy, and length. As these methods are frequently accompanied by an analysis of cell proliferation and cell viability, some of these approaches are also described.
细胞定量广泛应用于基础研究和应用研究。其应用的一个典型例子是药物发现研究。目前，有大量的细胞定量方法。在这篇综述中，描述了用于细胞定量的基本技术，特别强调了基于荧光 DNA 染料的技术。本文的主要目的是引导读者了解各种方法进行细胞定量的可能性，并展示这些方法的优缺点，特别是在灵敏度、准确性和耗时方面。由于这些方法通常伴随着细胞增殖和细胞活力的分析，因此也描述了其中的一些方法。
