Leblond P, Demuyter P, Moutier L, Laakel M, Decaris B, Simonet J M
Laboratoire de Génétique et Microbiologie, Faculté des Sciences de l'Université de Nancy I, France.
J Bacteriol. 1989 Jan;171(1):419-23. doi: 10.1128/jb.171.1.419-423.1989.
The wild-type strain Streptomyces ambofaciens DSM 40697 exhibits a high degree of genetic instability. Pigment-defective colonies were observed in the progeny of wild-type colonies at a frequency of about 0.01. While only 13% of these pigment-defective colonies gave rise to homogeneous progeny exhibiting the mutant parental phenotype, 87% of the mutant colonies gave rise to hetergeneous progeny without a preponderant phenotype. This new phenomenon of instability was called hypervariability. In addition, 21% of the mutant strains arising in hypervariable progeny contained highly reiterated DNA sequences, while amplified DNA sequences could be detected in neither stable pigment-defective mutant clones nor in wild-type clones. These results indicate a frequent association between genetic instability and hypervariability and a frequent association between hypervariability and amplification of DNA sequences.
野生型菌株浅青紫链霉菌DSM 40697表现出高度的遗传不稳定性。在野生型菌落的子代中观察到色素缺陷型菌落，频率约为0.01。虽然这些色素缺陷型菌落中只有13%产生了表现出突变亲本表型的同质子代，但87%的突变菌落产生了没有优势表型的异质子代。这种新的不稳定性现象被称为高变异性。此外，在高变异性子代中产生的突变菌株中有21%含有高度重复的DNA序列，而在稳定的色素缺陷型突变克隆和野生型克隆中均未检测到扩增的DNA序列。这些结果表明遗传不稳定性与高变异性之间频繁相关，以及高变异性与DNA序列扩增之间频繁相关。
