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丝状原绿球藻(Prochlorothrix hollandica)细胞壁中的肽聚糖 - 多糖复合物。

Peptidoglycan-polysaccharide complex in the cell wall of the filamentous prochlorophyte Prochlorothrix hollandica.

作者信息

Jürgens U J, Burger-Wiersma T

机构信息

Institut für Biologie II, Albert-Ludwigs-Universität, Freiburg im Breisgau, Federal Republic of Germany.

出版信息

J Bacteriol. 1989 Jan;171(1):498-502. doi: 10.1128/jb.171.1.498-502.1989.

DOI:10.1128/jb.171.1.498-502.1989
PMID:2914854
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC209614/
Abstract

A peptidoglycan-polysaccharide complex composed of N-acetylglucosamine, N-acetylmuramic acid, muramic acid 6-phosphate, L-alanine, D-alanine, D-glutamic acid, meso-diaminopimelic acid, N-acetylmannosamine, mannose, galactose, glucose, and phosphate was isolated from cell walls of the filamentous prochlorophyte Prochlorothrix hollandica; this complex was similar in chemical composition and structure to that found in cyanobacteria. Peptide patterns of partial acid hydrolysates of the isolated peptidoglycan revealed an A1 gamma structure with direct cross-linkage (m-diaminopimelic acid-D-alanine) of the peptide side chains. The degree of cross-linkage (63%) was found to be in the range of values obtained for gram-positive bacteria and cyanobacteria.

摘要

从丝状原绿藻荷兰原绿球藻的细胞壁中分离出一种由N - 乙酰葡糖胺、N - 乙酰胞壁酸、胞壁酸6 - 磷酸、L - 丙氨酸、D - 丙氨酸、D - 谷氨酸、内消旋二氨基庚二酸、N - 乙酰甘露糖胺、甘露糖、半乳糖、葡萄糖和磷酸盐组成的肽聚糖 - 多糖复合物;该复合物在化学组成和结构上与蓝细菌中的类似。分离出的肽聚糖部分酸水解产物的肽图谱显示出肽侧链具有直接交联(内消旋二氨基庚二酸 - D - 丙氨酸)的A1γ结构。发现交联度(63%)处于革兰氏阳性菌和蓝细菌所获得的值范围内。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a669/209614/9d64f293bf3f/jbacter00167-0522-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a669/209614/1eda3c885bf6/jbacter00167-0521-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a669/209614/9d64f293bf3f/jbacter00167-0522-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a669/209614/1eda3c885bf6/jbacter00167-0521-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a669/209614/9d64f293bf3f/jbacter00167-0522-a.jpg

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本文引用的文献

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Supramolecular structure of stacked and unstacked regions of the photosynthetic membranes of Prochloron sp., a prokaryote.原绿球藻(一种原核生物)光合膜堆叠区和非堆叠区的超分子结构。
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Primary structure of the peptidoglycan from the unicellular cyanobacterium Synechocystis sp. strain PCC 6714.单细胞蓝藻集胞藻6714菌株肽聚糖的一级结构
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Fluorescamine: a reagent for assay of amino acids, peptides, proteins, and primary amines in the picomole range.荧光胺:一种用于测定皮摩尔范围内氨基酸、肽、蛋白质和伯胺的试剂。
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