Strøm H K, Ohtani M, Nowak B, Boutrup T S, Jones B, Raida M K, Bojesen A M
Section of Veterinary Clinical Microbiology, Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Frederiksberg, Denmark.
Institute for Marine and Antarctic Studies, University of Tasmania, Launceston, Tas., Australia.
J Fish Dis. 2018 Mar;41(3):529-537. doi: 10.1111/jfd.12754. Epub 2017 Nov 17.
Pathological manifestations in rainbow trout (Oncorhynchus mykiss) following experimental waterborne infection with Yersinia ruckeri serotype O1 biotype 2 (strain 07111224) were investigated. Rainbow trout were exposed to 8 × 10 CFU/ml of Y. ruckeri by bath for 6 hr, and mortality was then monitored for 22 days post-infection (dpi). Organs were sampled at 3 dpi and also from moribund fish showing signs of severe systemic infection such as bleeding, exophthalmia or erratic swimming behaviour. Y. ruckeri was observed in the meninges and diencephalon of the brain, and lamina propria of olfactory organ at 3 dpi. At 12 dpi, Y. ruckeri had spread throughout the brain including cranial connective tissues and ventricles and the infection was associated with haemorrhages and an infiltration with leucocytes. Y. ruckeri infection and associated with leucocyte infiltration were observed at 13 dpi. In conclusion, Y. ruckeri strain 07111224 causes encephalitis in the acute phase of infection, which could explain why Y. ruckeri-affected fish show exophthalmia and erratic swimming known as signs of ERM.
研究了虹鳟（Oncorhynchus mykiss）经实验性水传播感染鲁氏耶尔森菌血清型O1生物型2（菌株07111224）后的病理表现。将虹鳟通过浸浴暴露于8×10⁶CFU/ml的鲁氏耶尔森菌中6小时，然后在感染后22天（dpi）监测死亡率。在3 dpi时对器官进行采样，同时也从出现严重全身感染迹象（如出血、眼球突出或游泳行为异常）的濒死鱼中采样。在3 dpi时，在脑的脑膜和间脑以及嗅觉器官的固有层中观察到鲁氏耶尔森菌。在12 dpi时，鲁氏耶尔森菌已扩散至整个脑部，包括颅结缔组织和脑室，并且感染与出血以及白细胞浸润有关。在13 dpi时观察到鲁氏耶尔森菌感染并伴有白细胞浸润。总之，鲁氏耶尔森菌菌株07111224在感染急性期引起脑炎，这可以解释为什么受鲁氏耶尔森菌感染的鱼会出现眼球突出和游泳行为异常，即所谓的ERM症状。
