一种基于酶解的新型 UPLC-MS/MS 方法用于鉴定和区分麻黄属多糖。
A New UPLC-MS/MS Method for the Characterization and Discrimination of Polysaccharides from Genus Ephedra Based on Enzymatic Digestions.
机构信息
Key Laboratory of Chinese Materia Medica, Heilongjiang University of Chinese Medicine, Ministry of Education, Harbin 150040, China.
出版信息
Molecules. 2017 Nov 17;22(11):1992. doi: 10.3390/molecules22111992.
polysaccharides have been reported to possess important activities, so quality evaluation of polysaccharides from the genus is urgent. In this study, enzymatic digestions were performed to establish multiple saccharide fingerprints by ultra-performance liquid chromatography with electrospray ionization triple quadrupole linear ion trap mass spectrometry (UPLC-ESI-TQ-MS/MS) based on a multiple-reaction monitoring in negative mode. Under optimum UPLC-ESI⁻-TQ-MS/MS conditions, excellent separation and quantification of 21 constituents were achieved within 20 min on a solid core column with a 1.6 μm particle using pre-column derivatization with a PMP reagent. This method, coupled with enzymatic digestions and principal component analysis, has been successfully applied to characterize and discriminate polysaccharides attributed to different species and plant parts. The results suggest that the proposed analytical strategy could achieve a quality evaluation of plant polysaccharides from traditional Chinese medicines.
多糖被报道具有重要的活性，因此迫切需要对该属的多糖进行质量评价。在这项研究中，通过超高效液相色谱-电喷雾电离三重四极杆线性离子阱质谱（UPLC-ESI-TQ-MS/MS）在负离子模式下进行酶解，建立了多个糖指纹图谱。在最佳的 UPLC-ESI⁻-TQ-MS/MS 条件下，使用 PMP 试剂进行柱前衍生，在 1.6μm 粒径的实心核柱上，在 20 min 内可实现 21 种成分的出色分离和定量。该方法结合酶解和主成分分析，已成功应用于表征和区分不同种属和植物部位的多糖。结果表明，所提出的分析策略可实现对中药植物多糖的质量评价。
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