Guo Bing, Yang Lina, Li Hengyang, An Qi, Liu Yongli, Cheng Jie, Hou Fangjie, Guo Long, Zhang Dan
Traditional Chinese Medicine Processing Technology Innovation Centre of Hebei Province, College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, China.
International Joint Research Centre on Resource Utilization and Quality Evaluation of Traditional Chinese Medicine of Hebei Province, College of Pharmacy, Hebei University of Chinese Medicine, Shijiazhuang, China.
Front Plant Sci. 2024 Jun 12;15:1421008. doi: 10.3389/fpls.2024.1421008. eCollection 2024.
, widely used in clinical practice as a medicinal herb, belongs to the genus in the family Ephedraceae. However, the presence of numerous varieties and variants requires differentiation for accurate identification.
In this study, we employed headspace gas chromatography mass spectrometry (HS-GC-MS), ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS), and global natural products social molecular networking (GNPS) for chemical component identification. Chemometric analysis was used to analyze the differential components. Metabolic analysis and Kyoto encyclopedia of genes and genomes (KEGG) enrichment were utilized to explore the synthesis pathways of different components.
A total of 83 volatile and 79 non-volatile components were identified in species. Differential analysis revealed that among the eight stems, 18 volatile and 19 non-volatile differential compounds were discovered, whereas roots exhibited 21 volatile and 17 non-volatile markers. Volatile compounds were enriched in four synthetic pathways, while non-volatile components were enriched in five pathways among the differentiated components.
This study is the first to conduct a comparative analysis of chemical components in different species and parts. It provides a foundational reference for authenticating herbs, evaluating medicinal resources, and comparing quality in future studies.
作为一种草药在临床实践中广泛应用，属于麻黄科麻黄属。然而，众多的品种和变种需要进行区分以实现准确鉴定。
在本研究中，我们采用顶空气相色谱 - 质谱联用（HS - GC - MS）、超高效液相色谱与四极杆飞行时间质谱联用（UPLC - Q - TOF - MS）以及全球天然产物社会分子网络（GNPS）进行化学成分鉴定。化学计量学分析用于分析差异成分。代谢分析和京都基因与基因组百科全书（KEGG）富集分析用于探索不同成分的合成途径。
在该物种中总共鉴定出83种挥发性成分和79种非挥发性成分。差异分析表明，在八个麻黄茎中，发现了18种挥发性和19种非挥发性差异化合物，而麻黄根表现出21种挥发性和17种非挥发性标志物。在差异成分中，挥发性化合物富集于四条合成途径，而非挥发性成分富集于五条途径。
本研究首次对不同麻黄物种和部位的化学成分进行了比较分析。它为未来研究中鉴定麻黄药材、评估药用资源和比较质量提供了基础参考。
