He Yu, Zhu Ying, Zhang Ruping, Ge Lijun, Wan Haitong
Department of Medicinal Chemistry, College of Pharmaceutical Science, Zhejiang Chinese Medical University, Hangzhou, People's Republic of China.
College of Bioengineering, Zhejiang Chinese Medical University, Hangzhou, People's Republic of China.
Pharmacogn Mag. 2014 Jan;10(Suppl 1):S72-9. doi: 10.4103/0973-1296.127346.
Mahuang decoction (MHD), a famous classic traditional Chinese formula, has been extensively applied for treating cold, influenza, asthma, acute bronchitis, and other pulmonary diseases. However, the interaction among four drugs of MHD has not been clearly deciphered from the aspect of molecular composition.
To assess the quality of MHD and explore the interplay among different prescription drugs.
A reversed-phase high performance liquid chromatography (RP-HPLC) coupled with diode array detector (DAD) method for the simultaneous separation and determination of nine bioactive components was developed. A somatomedin A (SMA)-phenyl column (4.6 mm × 250 mm, 5 μm) was eluted by a gradient mobile phase contained acetonitrile and 0.05% formic acid-0.05% triethylamine aqueous solution. Four detection wavelengths (210, 252, 278, and 291 nm) were utilized for the quantitative analysis due to the different ultraviolet (UV) spectra of these compounds.
Satisfactory separation was obtained for all the components, and the assay was fully validated in respects of linearity, precision, stability, and accuracy. It was found that the calibration curves for all analytes showed good linearity (R (2)≥ 0.9991) within the test ranges. The relative standard deviations (RSDs) for intra- and interday repeatability were not more than 1.70 and 2.66%, respectively. The spike recoveries of nine components varied from 97.50 ± 1.69 to 99.27 ± 1.37%.
The established method was successfully applied to analyze nine active compounds in decoction samples of various drug compatibilities of MHD. The variations of contents were obvious for different combinations, which hinted the mutual promotion or inhibition of componential dissolution among four herbs of MHD.
麻黄汤是著名的经典中药方剂，已广泛应用于治疗感冒、流感、哮喘、急性支气管炎及其他肺部疾病。然而，从分子组成方面尚未明确解读麻黄汤四味药之间的相互作用。
评估麻黄汤的质量并探究不同处方药之间的相互作用。
建立了一种反相高效液相色谱（RP-HPLC）结合二极管阵列检测器（DAD）的方法，用于同时分离和测定9种生物活性成分。采用生长激素介质A（SMA）-苯基柱（4.6 mm×250 mm，5μm），以含乙腈和0.05%甲酸-0.05%三乙胺水溶液的梯度流动相进行洗脱。由于这些化合物的紫外（UV）光谱不同，因此采用四个检测波长（210、252、278和291 nm）进行定量分析。
所有成分均获得满意的分离效果，该测定方法在线性、精密度、稳定性和准确性方面均得到充分验证。结果发现，所有分析物的校准曲线在测试范围内均显示出良好的线性关系（R²≥0.9991）。日内和日间重复性的相对标准偏差（RSD）分别不超过1.70%和2.66%。9种成分的加样回收率在97.50±1.69%至99.27±1.37%之间。
所建立的方法成功应用于分析麻黄汤不同药物配伍煎剂样品中的9种活性成分。不同组合的含量变化明显，这提示麻黄汤四味药之间成分溶解存在相互促进或抑制作用。
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