Department of Biological Sciences, Faculty of Science, National University of Singapore, Singapore, 117543, Republic of Singapore.
Temasek Life Sciences Laboratory, 1 Research Link, National University of Singapore, Singapore, 117604, Republic of Singapore.
Plant Cell Rep. 2018 Feb;37(2):377-385. doi: 10.1007/s00299-017-2235-8. Epub 2017 Nov 17.
Ds insertion in rice OsPS1-F gene results in semi-dwarf plants with reduced tiller number and grain yield, while genetic complementation with OsPS1-F rescued the mutant phenotype. Photosynthetic electron transport is regulated in the chloroplast thylakoid membrane by multi-protein complexes. Studies about photosynthetic machinery and its subunits in crop plants are necessary, because they could be crucial for yield enhancement in the long term. Here, we report the characterization of OsPS1-F (encoding Oryza sativa PHOTOSYSTEM 1-F subunit) using a single copy Ds insertion rice mutant line. The homozygous mutant (osps1-f) showed striking difference in growth and development compared to the wild type (WT), including, reduction in plant height, tiller number, grain yield as well as pale yellow leaf coloration. Chlorophyll concentration and electron transport rate were significantly reduced in the mutant compared to the WT. OsPS1-F gene was highly expressed in rice leaves compared to other tissues at different developmental stages tested. Upon complementation of the mutant with proUBI::OsPS1-F, the observed mutant phenotypes were rescued. Our results illustrate that OsPS1-F plays an important role in regulating proper growth and development of rice plants.
Ds 插入水稻 OsPS1-F 基因导致分蘖数和产量减少的半矮秆植物，而 OsPS1-F 的遗传互补挽救了突变体表型。光合作用电子传递在叶绿体类囊体膜中由多蛋白复合物调节。研究作物中光合作用机器及其亚基是必要的，因为它们可能对长期提高产量至关重要。在这里，我们使用单个拷贝 Ds 插入水稻突变体系来描述 OsPS1-F（编码 Oryza sativa PHOTOSYSTEM 1-F 亚基）的特征。与野生型（WT）相比，纯合突变体（osps1-f）在生长和发育方面表现出明显的差异，包括株高、分蘖数、粒产量以及叶片呈淡黄色。与 WT 相比，突变体中的叶绿素浓度和电子传递率显著降低。与其他组织相比，OsPS1-F 基因在不同发育阶段的水稻叶片中高度表达。在突变体中用 proUBI::OsPS1-F 进行互补后，观察到的突变体表型得到挽救。我们的结果表明，OsPS1-F 在调节水稻植株的正常生长和发育中起着重要作用。
