Anatomisches Institut, Anatomie & Zellbiologie, Medical Faculty of the University of Bonn, Nussallee 10, 53115, Bonn, Germany.
Institut für Zelluläre Neurowissenschaften, Medical Faculty of the University of Bonn, Bonn, Germany.
Cerebellum. 2018 Feb;17(1):62-71. doi: 10.1007/s12311-017-0900-7.
The cerebellum arguably constitutes one of the best characterized central nervous circuits, and its structure, cellular function, and histogenesis have been described in exceptional quantitative detail. A notable exception to this is the development of its inhibitory interneurons, and in particular the extensive migrations of future basket and stellate cells. Here, we used acute slices from 8-day-old mice to assess the migration of Pax2-EGFP-tagged precursors of these cells en route to the molecular layer during their transit through the nascent cerebellar cortex. We document that movement of these cells is highly directed. Their speed and directional persistence are larger in the nascent granule cell layer than in the molecular layer. And they migrate periodically, with periods of effective, directed translocation separated by bouts of rather local movement. Finally, we document that the arrangement of these cells in the adult molecular layer is characterized by clustering. These data are discussed with a focus on potential generative mechanisms for the developmental pattern observed.
小脑可以说是中枢神经系统中特征最为明确的回路之一，其结构、细胞功能和组织发生都已得到了非常详细的描述。但有一个显著的例外，那就是其抑制性中间神经元的发育，特别是未来篮状细胞和星状细胞的广泛迁移。在这里，我们使用来自 8 天大的小鼠的急性脑片，在它们通过新生小脑皮层到达分子层的过程中，评估这些细胞的 Pax2-EGFP 标记前体的迁移。我们证明了这些细胞的运动具有高度的方向性。它们在新生颗粒细胞层中的速度和方向持久性大于在分子层中的速度和方向持久性。并且它们周期性地迁移，有效、定向的转位周期与相当局部的运动周期交替。最后，我们证明这些细胞在成年分子层中的排列特征是聚类。这些数据将与观察到的发育模式的潜在生成机制一起进行讨论。
