Advanced Water Management Centre, The University of Queensland, St Lucia, Brisbane, QLD 4072, Australia; Department of Environmental Science & Engineering, Sichuan University, Chengdu, Sichuan 610065, China.
Advanced Water Management Centre, The University of Queensland, St Lucia, Brisbane, QLD 4072, Australia.
Water Res. 2018 Feb 1;129:163-171. doi: 10.1016/j.watres.2017.11.021. Epub 2017 Nov 8.
The intensive use of silver nanoparticles (AgNPs) in cosmetics and textiles causes their release into sewer networks of urban water systems. Although a few studies have investigated antimicrobial activities of nanoparticles against environmental bacteria, little is known about potential impacts of the released AgNPs on sulfate reducing bacteria in sewers. Here, we investigated the effect of AgNPs on Desulfovibrio vulgaris Hidenborough (D. vulgaris), a typical sulfate-reducing bacterium (SRB) in sewer systems. We found AgNPs stimulated the proliferation of D. vulgaris, rather than exerting inhibitory or biocidal effects. Based on flow cytometer detections, both the cell growth rate and the viable cell ratio of D. vulgaris increased during exposure to AgNPs at concentrations of up to 100 mg/L. The growth stimulation was dependent on the AgNP concentration. These results imply that the presence of AgNPs in sewage may affect SRB abundance in sewer networks. Our findings also shed new lights on the interactions of nanoparticles and bacteria.
纳米银颗粒（AgNPs）在化妆品和纺织品中的大量使用会导致它们释放到城市水系统的污水管网中。尽管有一些研究调查了纳米颗粒对环境细菌的抗菌活性，但对于释放的 AgNPs 对污水中硫酸盐还原菌（SRB）的潜在影响知之甚少。在这里，我们研究了 AgNPs 对脱硫弧菌（Desulfovibrio vulgaris Hidenborough，D. vulgaris）的影响，脱硫弧菌是污水系统中一种典型的硫酸盐还原菌（SRB）。我们发现 AgNPs 刺激了 D. vulgaris 的增殖，而不是产生抑制或杀菌作用。基于流式细胞仪检测，在浓度高达 100mg/L 的 AgNPs 暴露下，D. vulgaris 的细胞生长率和活细胞比例均增加。这种生长刺激依赖于 AgNP 的浓度。这些结果表明，污水中存在 AgNPs 可能会影响污水管网中 SRB 的丰度。我们的研究结果还为纳米颗粒和细菌的相互作用提供了新的认识。
