Cheptsov V S, Tsypina S I, Minaev N V, Yusupov V I, Chichkov B N
Department of Soil Science, Lomonosov Moscow State University, 11999 Moscow, Russia.
Research Center "Crystallography and Photonics" RAS, Institute of Photonic Technologies, Troitsk, Moscow, Russia.
Int J Bioprint. 2018 Dec 14;5(1):165. doi: 10.18063/ijb.v5i1.165. eCollection 2019.
The study of biodiversity, growth, development, and metabolism of cultivated microorganisms is an integral part of modern microbiological, biotechnological, and medical research. Such studies require the development of new methods of isolation, cultivation, manipulation, and study of individual bacterial cells and their consortia. To this end, in recent years, there has been an active development of different isolation and three-dimensional cell positioning methods. In this review, the optical tweezers, surface heterogeneous functionalization, multiphoton lithography, microfluidic techniques, and laser printing are reviewed. Laser printing is considered as one of the most promising techniques and is discussed in detail.
对培养微生物的生物多样性、生长、发育和代谢的研究是现代微生物学、生物技术和医学研究不可或缺的一部分。此类研究需要开发新的方法来分离、培养、操控和研究单个细菌细胞及其群落。为此，近年来，不同的分离和三维细胞定位方法得到了积极发展。在本综述中，对光镊、表面异质功能化、多光子光刻、微流控技术和激光打印进行了综述。激光打印被认为是最具前景的技术之一，并进行了详细讨论。
