The Francis Crick Institute, London NW1 1AT, UK.
The Francis Crick Institute, London NW1 1AT, UK; Imperial College, London W12 0NN, UK.
Curr Opin Cell Biol. 2018 Apr;51:8-14. doi: 10.1016/j.ceb.2017.10.002. Epub 2017 Nov 14.
The number of mature B cells is carefully controlled by signalling from receptors that support B cell survival. The best studied of these are the B cell antigen receptor (BCR) and BAFFR. Recent work has shown that signalling from these receptors is closely linked, involves the CD19 co-receptor, and leads to activation of canonical and non-canonical NF-κB pathways, ERK1, ERK2 and ERK5 MAP kinases, and PI-3 kinases. Importantly, studies show that investigation of the importance of signalling molecules in cell survival requires the use of inducible gene deletions within mature B cells. This overcomes the limitations of many earlier studies using constitutive gene deletions which were unable to distinguish between requirements for a protein in development versus survival.
成熟 B 细胞的数量受到支持 B 细胞存活的受体信号的精细控制。其中研究得最好的是 B 细胞抗原受体 (BCR) 和 BAFFR。最近的研究表明，这些受体的信号传导密切相关，涉及 CD19 共受体，并导致经典和非经典 NF-κB 途径、ERK1、ERK2 和 ERK5 MAP 激酶以及 PI-3 激酶的激活。重要的是，研究表明，研究信号分子在细胞存活中的重要性需要在成熟 B 细胞中使用诱导型基因缺失。这克服了许多早期使用组成型基因缺失的研究的局限性，这些研究无法区分蛋白质在发育和存活中的需求。
