Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia; Saratov Science Research Veterinary Institute, Russian Academy of Sciences, 6 Ulitsa 53-ei Strelkovoi Divizii, Saratov 410028, Russia.
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 13 Prospekt Entuziastov, Saratov 410049, Russia; Saratov Science Research Veterinary Institute, Russian Academy of Sciences, 6 Ulitsa 53-ei Strelkovoi Divizii, Saratov 410028, Russia.
Int Immunopharmacol. 2018 Jan;54:163-168. doi: 10.1016/j.intimp.2017.11.008. Epub 2017 Nov 14.
Gold nanoparticles (GNPs) are advantageous as an adjuvant in the design of effective vaccines and in the preparation of high-affinity antibodies to haptens and complete antigens. Another method of activating immunocompetent cells with colloidal gold is to conjugate GNPs with CpG oligodeoxynucleotides (ODNs). We examined how the size and shape of GNPs and various combinations of GNPs and CpG ODNs 1826 affect the immune response. When animals were injected with a model antigen (BSA) coupled to gold nanospheres (diameters, 15 and 50nm), nanorods, nanoshells, and nanostars, the titers of the resultant antibodies differed substantially. The antibody titers decreased in the sequence GNPs-50nm>GNPs-15nm>nanoshells>nanostars>nanorods>native BSA. We conclude that 50 and 15nm gold nanospheres are the optimal antigen carrier and adjuvant for immunization. The highest titer of anti-BSA antibodies was detected in the blood serum of mice immunized simultaneously with BSA-GNP and CpG-GNP conjugates.
金纳米粒子（GNPs）作为一种有效的疫苗设计的佐剂和半抗原和完全抗原的高亲和力抗体的制备是有利的。用胶体金激活免疫活性细胞的另一种方法是将 GNPs 与 CpG 寡脱氧核苷酸（ODNs）偶联。我们研究了 GNPs 的大小和形状以及 GNPs 和 CpG ODNs 1826 的各种组合如何影响免疫反应。当用模型抗原（BSA）与金纳米球（直径 15 和 50nm）、纳米棒、纳米壳和纳米星偶联物给动物注射时，所得抗体的效价有很大差异。抗体效价依次降低：GNPs-50nm>GNPs-15nm>纳米壳>纳米星>纳米棒>天然 BSA。我们得出结论，50nm 和 15nm 金纳米球是免疫接种的最佳抗原载体和佐剂。在同时用 BSA-GNP 和 CpG-GNP 缀合物免疫的小鼠血清中检测到最高效价的抗 BSA 抗体。
