Pavan T R, Di Domenico J, Kirsten K S, Nied C O, Frandoloso R, Kreutz L C
Programa de Pós-Graduação em Bioexperimentação, Universidade de Passo Fundo, Passo Fundo, RS, Brasil.
Braz J Med Biol Res. 2016 Jul 25;49(8). doi: 10.1590/1414-431X20165281.
Adjuvants are essential to boost the immune response to inoculated antigen and play a central role in vaccine development. In this study, we investigated the efficacy of several adjuvants in the production of anti-bovine serum albumin (BSA) antibodies in silver catfish. Two hundred and seventy juvenile silver catfish (60-80 g) of both sexes were intraperitoneally vaccinated with BSA (200 µg/fish) alone or mixed to the following adjuvants: Freund's complete adjuvant (FCA), Freund's incomplete adjuvant (FIA), aluminum hydroxide (AlOH), Montanide, four types of cytosine-phosphate-guanine (CpG) oligodeoxynucleotides (ODNs) and three concentrations of β-glucan, and the immune enhancing property was evaluated by measuring anti-BSA antibodies in blood samples at biweekly intervals. Our results demonstrated that CpGs ODNs and β-glucan were as effective as classical adjuvants (FCA, FIA, AlOH and Montanide) in promoting anti-BSA antibodies and that the kinetics of antibody production induced by all adjuvants used in our study had a similar trend to that observed in other fish species, with a peak at 28 days post-vaccination. These results may be useful for the selection of adjuvants for vaccine formulation intended for silver catfish and for the development of vaccine and vaccination strategies to other fish species.
佐剂对于增强对接种抗原的免疫反应至关重要，并且在疫苗开发中发挥核心作用。在本研究中，我们调查了几种佐剂在斑点叉尾鮰体内产生抗牛血清白蛋白（BSA）抗体方面的功效。将270尾雌雄斑点叉尾鮰幼鱼（60 - 80克）分别单独腹腔接种BSA（200微克/尾），或与以下佐剂混合接种：弗氏完全佐剂（FCA）、弗氏不完全佐剂（FIA）、氢氧化铝（AlOH）、Montanide、四种胞嘧啶-磷酸-鸟嘌呤（CpG）寡脱氧核苷酸（ODN）以及三种浓度的β-葡聚糖，通过每两周检测一次血液样本中的抗BSA抗体来评估免疫增强特性。我们的结果表明，CpG ODN和β-葡聚糖在促进抗BSA抗体产生方面与传统佐剂（FCA、FIA、AlOH和Montanide）同样有效，并且我们研究中使用的所有佐剂诱导抗体产生的动力学与在其他鱼类中观察到的趋势相似，在接种后28天达到峰值。这些结果可能有助于为斑点叉尾鮰疫苗配方选择佐剂，以及为其他鱼类开发疫苗和接种策略。
