Nguyen Thao Duy, Prykhodko Olena, Fåk Hållenius Frida, Nyman Margareta
Food for Health Science Centre, Lund University, PO Box 124, SE-221 00, Lund, Sweden.
J Nutr Sci. 2017 Oct 11;6:e51. doi: 10.1017/jns.2017.54. eCollection 2017.
Butyric acid has been shown to have suppressive effects on inflammation and diseases related to the intestinal tract. The aim of the present study was to investigate whether supplementation of two glycerol esters, monobutyrin (MB) and tributyrin (TB), would reach the hindgut of rats, thus having an effect on the caecal profile of SCFA, microbiota composition and some risk markers associated with chronic inflammation. For this purpose, rats were fed high-fat diets after adding MB (1 and 5 g/kg) and TB (5 g/kg) to a diet without any supplementation (high-fat control; HFC). A low-fat (LF) diet was also included. In the liver, total cholesterol concentrations, LDL-cholesterol concentrations, LDL:HDL ratio, and succinic acid concentrations were reduced in rats given the MB and TB (5 g/kg) diets, compared with the group fed the HFC diet. These effects were more pronounced in MB than TB groups as also expressed by down-regulation of the gene . The composition of the caecal microbiota in rats fed MB and TB was separated from the group fed the HFC diet, and also the LF diet, as evidenced by the absence of the phylum TM7 and reduced abundance of the genera (similar to LF-fed rats) and . Notably, the caecal abundance of was markedly increased in the MB group compared with the HFC group. The results suggest that dietary supplementation of MB and TB can be used to counteract disturbances associated with a HFC diet, by altering the gut microbiota, and decreasing liver lipids and succinic acid concentrations.
丁酸已被证明对炎症及与肠道相关的疾病具有抑制作用。本研究的目的是调查补充两种甘油酯，即单丁酸甘油酯（MB）和三丁酸甘油酯（TB），是否能到达大鼠的后肠，从而对盲肠短链脂肪酸（SCFA）谱、微生物群组成以及一些与慢性炎症相关的风险标志物产生影响。为此，在不添加任何物质的高脂饮食（高脂对照；HFC）中添加MB（1和5 g/kg）和TB（5 g/kg）后喂养大鼠。还纳入了低脂（LF）饮食。在肝脏中，与喂食HFC饮食的组相比，喂食MB和TB（5 g/kg）饮食的大鼠的总胆固醇浓度、低密度脂蛋白胆固醇浓度、低密度脂蛋白：高密度脂蛋白比值以及琥珀酸浓度均降低。这些作用在MB组比TB组更明显，这也通过基因下调得以体现。喂食MB和TB的大鼠盲肠微生物群组成与喂食HFC饮食的组以及LF饮食的组不同，这可通过TM7门的缺失以及属（与喂食LF饮食的大鼠相似）和属的丰度降低来证明。值得注意的是，与HFC组相比，MB组中属的盲肠丰度显著增加。结果表明，通过改变肠道微生物群以及降低肝脏脂质和琥珀酸浓度，饮食中补充MB和TB可用于对抗与HFC饮食相关的干扰。
