National Field Station of Freshwater Ecosystem of Liangzi Lake, College of Life Sciences, Wuhan University, Wuhan, PR China.
National Field Station of Freshwater Ecosystem of Liangzi Lake, College of Life Sciences, Wuhan University, Wuhan, PR China.
Chemosphere. 2018 Feb;193:385-393. doi: 10.1016/j.chemosphere.2017.10.173. Epub 2017 Nov 2.
Glyphosate is a broad-spectrum herbicide that is frequently detected in water bodies and is harmful to aquatic systems. We conducted an experiment to explore the ecological sensitivity of Hydrilla verticillata (L.f.) Royle and Vallisneria natans (Lour.) Hara to glyphosate. Our research focused on the physiological responses of H. verticillata and V. natans after exposure to various concentrations of glyphosate (0, 1, 10, 20, 30, 40, 50 and 80 mg/L) in hydroponic culture after one day (1D) and seven days (7D). The results show that after 1D, the soluble protein content of H. verticillata was significantly stimulated under low herbicide concentrations. Other indices for H. verticillata and V. natans had no remarkable changes at 1D. After 7D of treatment, the soluble protein content of H. verticillata showed no significant differences, while the malondialdehyde (MDA), pigment contents and catalase (CAT) activity significantly increased at low glyphosate concentrations. Guaiacol peroxidase (POD) activity in H. verticillata significantly increased with increasing herbicide concentrations. The chlorophyll a/b ratio of H. verticillata sharply decreased above 10 mg/L. For V. natans, soluble protein, chlorophyll a, and carotenoid content; and CAT activity declined significantly after glyphosate application, while other indicators showed no significant changes. Our results indicate that glyphosate concentrations from 0 to 80 mg/L can induce oxidative stress in H. verticillate and may impede metabolism processes for protein and pigments without causing oxidative stress in V. natans. Taken together, our results suggest that the sensitivity of H. verticillata to glyphosate exposure is higher than that of V. natans.
草甘膦是一种广谱除草剂，经常在水体中被检测到，对水生系统有害。我们进行了一项实验，以探讨水蕴草和苦草对草甘膦的生态敏感性。我们的研究重点是在水培培养中，水蕴草和苦草在暴露于不同浓度的草甘膦（0、1、10、20、30、40、50 和 80mg/L）1 天（1D）和 7 天后（7D）的生理反应。结果表明，在 1D 时，低浓度除草剂下，水蕴草的可溶性蛋白含量显著增加。水蕴草和苦草的其他指标在 1D 时没有明显变化。处理 7D 后，水蕴草的可溶性蛋白含量没有显著差异，而低浓度草甘膦下丙二醛（MDA）、色素含量和过氧化氢酶（CAT）活性显著增加。水蕴草的愈创木酚过氧化物酶（POD）活性随除草剂浓度的增加而显著增加。水蕴草的叶绿素 a/b 比在 10mg/L 以上急剧下降。对于苦草，草甘膦处理后可溶性蛋白、叶绿素 a 和类胡萝卜素含量以及 CAT 活性显著下降，而其他指标没有显著变化。我们的结果表明，0 至 80mg/L 的草甘膦浓度会引起水蕴草的氧化应激，可能会阻碍蛋白质和色素的代谢过程，但不会引起苦草的氧化应激。总的来说，我们的结果表明，水蕴草对草甘膦暴露的敏感性高于苦草。
