Lakkakula Jaya R, Ndinteh Derek Tantoh, van Vuuren Sandy F, Olivier Denise K, Krause Rui W M
Department of Bioscience and Bioengineering, IIT Bombay, Powai, Mumbai, India.
Centre for Nanomaterial Science Research, University of Johannesburg, PO Box 17011, Doornfontein, Johannesburg 2028, South Africa.
IET Nanobiotechnol. 2017 Dec;11(8):1017-1026. doi: 10.1049/iet-nbt.2017.0084.
The one-pot synthesis of silver nanoparticles (AgNPs) using the medium-polar extract of (Sw.) DC. is presented here as an alternative synthesis of metal NPs. Characterisation of the formed NPs showed polydispersed AgNPs ranging from 15 to 100 nm where the concentration of metal ions was found to play a role in the size and shape of the prepared NPs. It could be established that the flavonoids, saponins, and alkaloids present in the extract acted as both reducing and stabilising agents during the formation of the capped metal NPs. This means of NP synthesis was also employed during the in situ immobilisation of AgNPs on gauze and plaster. An evaluation of the antibacterial activity of the medium-polar . extract, AgNPs suspended in solution, and the immobilised AgNPs against (ATCC 25923), (ATCC 11778), and (ATCC 25922) showed high efficacy against the latter in particular. This suggests that gauze, dilute silver nitrate solutions, and extract could be used successfully in the simple in situ preparation of effective antibacterial wound dressings.
本文介绍了使用（Sw.）DC.的中极性提取物一锅法合成银纳米颗粒（AgNPs），作为金属纳米颗粒的一种替代合成方法。对形成的纳米颗粒的表征显示，多分散的AgNPs粒径范围为15至100 nm，发现金属离子浓度对所制备纳米颗粒的尺寸和形状有影响。可以确定，提取物中存在的黄酮类化合物、皂苷和生物碱在带帽金属纳米颗粒的形成过程中既充当还原剂又充当稳定剂。这种纳米颗粒合成方法也用于将AgNPs原位固定在纱布和膏药上。对中极性提取物、悬浮在溶液中的AgNPs以及固定化的AgNPs对金黄色葡萄球菌（ATCC 25923）、枯草芽孢杆菌（ATCC 11778）和大肠杆菌（ATCC 25922）的抗菌活性评估表明，尤其对后者具有高效性。这表明纱布、稀硝酸银溶液和提取物可以成功用于简单的原位制备有效的抗菌伤口敷料。
