a Faculty of Sciences, Department of Life Sciences, Laboratory of Biodiversity and Aquatic Ecosystems, Ecology and Planktonology , University of Sfax , Sfax , Tunisia.
b Faculty of Sciences, Department of life sciences, Laboratory of Animal Ecophysiology , University of Sfax , Sfax , Tunisia.
Arch Physiol Biochem. 2018 Jul;124(3):261-274. doi: 10.1080/13813455.2017.1395890. Epub 2017 Nov 20.
A total of five components (Catechin, Caffeic acid, Ferulic acid, Rosmarinic acid, and Amentoflavone) were identified in Periploca angustifolia leaf methanolic extract. This extract did not cause any cytotoxic effect on HepG2 cell line within the range of concentrations tested (0-400 µg mL). Thus, pre-treatment with 100 µg mL of P. angustifolia leaf methanolic extract (PAE) significantly (p < .05) protective HepG2 cells against cytotoxicity induced by cadmium exposure. However, Cd-intoxication significantly (p < .05) increased alanine and aspartate amino transferases serum activities (ALT and AST) and bilirubin content by 1.85-, 1.13-, and 3.55-fold, respectively. The levels of hepatic antioxidant parameters including superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were significantly (p < .05) decreased in Cd-intoxicated rats with concomitant enhancement of lipid peroxidation. Our results showed that P. angustifolia leaf methanolic extract can induce antioxidant effects and also exerts beneficial effects for the treatment of Cd-induced hepatotoxicity.
总共从杠柳叶甲醇提取物中鉴定出了 5 种成分（儿茶素、咖啡酸、阿魏酸、迷迭香酸和芹菜素）。在测试的浓度范围内（0-400μg/mL），该提取物对 HepG2 细胞系没有任何细胞毒性作用。因此，用 100μg/mL 的杠柳叶甲醇提取物（PAE）预处理可显著（p<.05）保护 HepG2 细胞免受镉暴露引起的细胞毒性。然而，镉中毒使血清中丙氨酸氨基转移酶（ALT）和天冬氨酸氨基转移酶（AST）以及胆红素含量分别增加了 1.85 倍、1.13 倍和 3.55 倍。同时，镉中毒大鼠肝抗氧化参数超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GPx）的水平显著降低（p<.05），脂质过氧化作用增强。我们的结果表明，杠柳叶甲醇提取物可诱导抗氧化作用，并对治疗镉诱导的肝毒性具有有益作用。
