Department of life sciences, Laboratory of Biodiversity and Aquatic Ecosystems, Ecology and plankktonology, University of Sfax Tunisia, Faculty of Sciences, Unit UR 11 ES 72/Street of Soukra Km 3,5, B.P. 1171, CP 3000 Sfax, Tunisia.
Department of life sciences, Laboratory of Biodiversity and Aquatic Ecosystems, Ecology and plankktonology, University of Sfax Tunisia, Faculty of Sciences, Unit UR 11 ES 72/Street of Soukra Km 3,5, B.P. 1171, CP 3000 Sfax, Tunisia.
Int J Biol Macromol. 2018 Mar;108:853-862. doi: 10.1016/j.ijbiomac.2017.10.175. Epub 2017 Oct 31.
The extraction of Periploca polysaccharides (PAPS) was optimized using the response surface methodology. The influence of solvent, liquid-solid ratio and extraction time on polysaccharide yield was evaluated using a full factorial design (2). Also, PAPS extract did not induce a cytotoxic effect on HepG2 cells within the range of tested concentrations (0-250μgmL). Herein, the pre-treatment with PAPS extract (100μgmL) reduced cell mortality. Furthermore, the in vivo antioxidant activity of PAPS extract was investigated in rats. The oral administration of 250mgkg body weight of PAPS extract administered above a period of 10 weeks to cadmium chloride (CdCl) induced toxicity in male Wistar rats, markedly decreased the content of MDA and protein damage in liver tissue, and enhanced liver function parameters (ALAT, ASAT and bilirubin), as well as the activities of hepatic antioxidant status (SOD, CAT, GPx and GSH). Finally, the examination of liver histopathology confirmed that PAPS ameliorate the alteration of liver tissue caused by exposition to cadmium.
采用响应面法优化了杠柳多糖（PAPS）的提取工艺。通过完全析因设计（2）评估了溶剂、液固比和提取时间对多糖得率的影响。此外，在所测试的浓度范围内（0-250μgmL），杠柳多糖提取物对 HepG2 细胞没有诱导细胞毒性作用。在此，PAPS 提取物（100μgmL）预处理可降低细胞死亡率。此外，还研究了杠柳多糖提取物在大鼠体内的抗氧化活性。在雄性 Wistar 大鼠中，给予氯化镉（CdCl）诱导毒性后，口服给予 250mgkg 体重的 PAPS 提取物 10 周以上，明显降低了肝组织中 MDA 和蛋白质损伤的含量，并增强了肝功能参数（ALAT、ASAT 和胆红素），以及肝抗氧化状态的活性（SOD、CAT、GPx 和 GSH）。最后，肝脏组织病理学检查证实，杠柳多糖可改善暴露于镉引起的肝组织改变。
