Department of Marine Food Science and Technology, Gangneung-Wonju National University, 120 Gangneungdaehangno, Gangneung, Gangwon 210-702, Republic of Korea.
Department of Marine Biotechnology, Gangneung-Wonju National University, 120 Gangneung, Gangwon 210-702, Republic of Korea.
Int J Biol Macromol. 2018 Mar;108:177-184. doi: 10.1016/j.ijbiomac.2017.11.102. Epub 2017 Nov 20.
The aqueous crude sulfated fucan (SF) from Stichopus japonicus was extracted and fractionated using anion-exchange chromatography to obtain four fractions (F, F, F and F) and to investigate their NK cell activation and cytotoxicity. The most potent NK cell cytotoxicity (45% at 250μg/mL) against HeLa cells was observed by F treatment, on the other hand, F and F treatment exhibited strong NK cell cytotoxicity (31-34% at 250μg/mL) against HepG2 and HT-29 cells. The SF treatment enhanced the activation of NK cells through the mRNA expression of IFN-γ, an activating receptor (NKp30), lysing proteins (perforin and granzyme-B) as well as a death ligand (FasL). However, the treatment of the SF derivatives, deproteinated-F and desulfated-F (DP-F and DS-F), markedly lowered the levels of NK cell cytotoxicity and mRNA expression of the activating factors, suggesting that the protein and sulfate were pivotal for the interaction between the SF and NK cells. The antibody neutralization test revealed that complement receptor-3 (CR3) may be a critical receptor involved in NK cell activation by the SF.
从海参中提取了粗制硫酸化岩藻聚糖（SF），并通过阴离子交换色谱对其进行了分离，得到了四个馏分（F、F、F 和 F），并研究了它们对 NK 细胞的激活和细胞毒性。F 处理对 HeLa 细胞的 NK 细胞毒性最强（250μg/mL 时为 45%），而 F 和 F 处理对 HepG2 和 HT-29 细胞的 NK 细胞毒性较强（250μg/mL 时为 31-34%）。SF 通过 IFN-γ（一种激活受体，NKp30）、裂解蛋白（穿孔素和颗粒酶-B）以及死亡配体（FasL）的 mRNA 表达，增强了 NK 细胞的激活。然而，SF 衍生物去蛋白-F 和去硫酸-F（DP-F 和 DS-F）的处理显著降低了 NK 细胞毒性和激活因子的 mRNA 表达水平，表明蛋白质和硫酸盐对于 SF 与 NK 细胞之间的相互作用至关重要。抗体中和试验表明，补体受体-3（CR3）可能是 SF 激活 NK 细胞的关键受体。
