Department of Marine Food Science and Technology, Gangneung-Wonju National University, 120 Gangneung, Gangwon 210-702, Republic of Korea; Department of Food Science and Technology, Souphanouvong University, 13th North Route Road, Ban Donemai, Luangprabang, Laos.
College of Maritime Studies and Management, Chiangmai University, Samut Sakhon 74000, Thailand.
Int J Biol Macromol. 2019 Feb 1;122:10-18. doi: 10.1016/j.ijbiomac.2018.10.119. Epub 2018 Oct 16.
The aqueous polysaccharide from Polygonatum sibiricum was extracted and fractionated using anion-exchange chromatography to obtain F fraction. The F was chemically sulfated and partially acid-hydrolyzed for the production of its over-sulfated (OS) and hydrolyzed (HP) derivatives, in which the sulfate content of OS was 7.5-17.1%, and the M of HP ranged from 18.2 × 10 to 57.3 × 10 g/mol. Considerable RAW264.7 cell activation was observed by HP with NO production of 34.9, 44.3 and 42.7 μM, respectively, as well as the mRNA expression of cytokines (IL-1β, IL-6, IL-10 and IL-12). NK cell cytotoxicity against HT-29 cell was facilitated by OS treatment with the increased gene expressions of INF-γ, Granzyme-B, perforin, NKG2D, and FasL. RAW264.7 cells appeared to be activated via MR and TLR4 mediated signaling pathway, but CR3 and TRL2 might play a main role in stimulating NK cells. Overall, the present study suggests the potential application of polysaccharides from P. sibiricum in functional foods and pharmacological industries.
西伯利亚百合水溶性多糖经阴离子交换色谱提取和分级，得到 F 级分。F 级分经化学硫酸化和部分酸水解制备其过度硫酸化（OS）和水解（HP）衍生物，其中 OS 的硫酸含量为 7.5-17.1%，HP 的 Mw 范围为 18.2×10 至 57.3×10 g/mol。HP 对 RAW264.7 细胞有明显的激活作用，NO 生成分别为 34.9、44.3 和 42.7 μM，以及细胞因子（IL-1β、IL-6、IL-10 和 IL-12）的 mRNA 表达。OS 处理促进 NK 细胞对 HT-29 细胞的细胞毒性，增加 IFN-γ、Granzyme-B、穿孔素、NKG2D 和 FasL 的基因表达。RAW264.7 细胞似乎通过 MR 和 TLR4 介导的信号通路被激活，但 CR3 和 TRL2 可能在刺激 NK 细胞中发挥主要作用。总之，本研究表明西伯利亚百合多糖在功能性食品和药理学工业中有潜在的应用。
