Chen Xiaopeng, Jiang Wen, Yue Chaofu, Zhang Wenjun, Tong Chaogang, Dai Dafei, Cheng Bin, Huang Chen, Lu Linming
Department of Hepatobiliary Surgery, Yijishan Hospital of Wannan Medical College, Wuhu 241001, China.
Department of General Surgery, Maanshan People's Hospital, Maanshan 243000, China.
J Cancer. 2017 Sep 16;8(16):3309-3317. doi: 10.7150/jca.20159. eCollection 2017.
The overall outcome of patients with hepatocellular carcinoma (HCC) is still very poor due to its high metastasis and recurrence rate. During metastasis, trans-endothelial migration (TEM) of HCC cells is a key step. Heparanase (HPSE) is an endo-beta-glucuronidase and exerts prometastatic properties for normal and tumor-derived cells. However, it is remains unclear that HPSE contributes to TEM of HCC cells. In this study, human umbilical vein endothelial cells-C (HUVEC-C) was used to simulate vascular endothelial cells (VECs), and the HCCLM3 cells with high HPSE expression were chosen and used for TEM assay and experiment. As results, we found that HCCLM3 cells showed higher TEM rate compared with other HCC cells. Downregulation or inhibition of HPSE activity resulted in suppression of TEM of HCC cells both and . Our findings suggest that HPSE contributes to TEM of HCC cells, which may be a new biological function of HPSE.
由于肝细胞癌（HCC）的高转移和复发率，其患者的总体预后仍然很差。在转移过程中，肝癌细胞的跨内皮迁移（TEM）是关键步骤。乙酰肝素酶（HPSE）是一种内切β-葡萄糖醛酸酶，对正常细胞和肿瘤来源细胞具有促转移特性。然而，HPSE是否有助于肝癌细胞的TEM尚不清楚。在本研究中，使用人脐静脉内皮细胞-C（HUVEC-C）模拟血管内皮细胞（VEC），并选择HPSE高表达的HCCLM3细胞用于TEM测定和实验。结果，我们发现HCCLM3细胞与其他肝癌细胞相比显示出更高的TEM率。HPSE活性的下调或抑制导致肝癌细胞的TEM均受到抑制。我们的研究结果表明，HPSE有助于肝癌细胞的TEM，这可能是HPSE的一种新生物学功能。
