Chatzidimitriou Konstantia, Gougoura Sofia G, Bargiota Alexandra, Koukoulis George N
Research Laboratory, Department of Endocrinology and Metabolic Diseases, Larissa University Hospital, School of Medicine, University of Thessaly, Biopolis, Larissa 41110, Greece.
J Clin Transl Endocrinol. 2015 Mar 18;2(2):61-65. doi: 10.1016/j.jcte.2015.03.001. eCollection 2015 Jun.
Plasma total adiponectin reveals a sexual dimorphism indicating that gonadal steroids may be involved in its secretion and/or metabolism. However, results from previous reports are conflicting and data regarding the influence of ovarian steroids on adiponectin's multimer forms are scarce. The objective of the study was to assess if total adiponectin and its isoforms are affected by the changes of estradiol and progesterone during the normal menstrual cycle and the association of total adiponectin and its isoforms with the gonadal steroid levels.
MATERIALS/METHODS: Quantitative determination of plasma adiponectin and its multimers was conducted in the three phases of an ovulatory cycle in 13 premenopausal women, in the follicular phase of 10 more premenopausal women, in 20 postmenopausal women and in 21 men. Moreover, serum levels of FSH, LH, prolactin, estradiol, progesterone, and testosterone, sex hormone binding globulin, glucose, and insulin were measured.
The circulating levels of total adiponectin and its multimers were not affected by the normal variation of estradiol and progesterone across the ovulatory menstrual cycle. In the whole number of participants, the total adiponectin and high molecular weight adiponectin levels were significantly different between genders and associated positively with age and sex hormone binding globulin levels, and negatively with testosterone and progesterone levels and the waist/hip ratio. In the multiple logistic regression analysis, after adjustment for age, gender, and sex hormone binding globulin and progesterone levels, significant predictors of total adiponectin levels were the waist/hip ratio and testosterone levels, and of high molecular weight adiponectin the testosterone levels.
Normal menstrual cycle ovarian steroids are not involved directly in the regulation of secretion and/or metabolism of total adiponectin and its multimers. Testosterone seems to be responsible for the adiponectin's sexual dimorphism.
血浆总脂联素呈现出性别差异，这表明性腺类固醇可能参与其分泌和/或代谢。然而，先前报告的结果相互矛盾，关于卵巢类固醇对脂联素多聚体形式影响的数据也很稀少。本研究的目的是评估在正常月经周期中，雌二醇和孕酮的变化是否会影响总脂联素及其异构体，以及总脂联素及其异构体与性腺类固醇水平之间的关联。
材料/方法：对13名绝经前女性排卵周期的三个阶段、另外10名绝经前女性的卵泡期、20名绝经后女性和21名男性的血浆脂联素及其多聚体进行了定量测定。此外，还测量了血清促卵泡激素（FSH）、促黄体生成素（LH）、催乳素、雌二醇、孕酮、睾酮、性激素结合球蛋白、葡萄糖和胰岛素的水平。
在排卵月经周期中，雌二醇和孕酮的正常变化并未影响总脂联素及其多聚体的循环水平。在所有参与者中，总脂联素和高分子量脂联素水平在性别之间存在显著差异，并且与年龄和性激素结合球蛋白水平呈正相关，与睾酮、孕酮水平以及腰臀比呈负相关。在多元逻辑回归分析中，在调整年龄、性别、性激素结合球蛋白和孕酮水平后，总脂联素水平的显著预测因素是腰臀比和睾酮水平，而高分子量脂联素的显著预测因素是睾酮水平。
正常月经周期的卵巢类固醇不直接参与总脂联素及其多聚体分泌和/或代谢的调节。睾酮似乎是脂联素性别差异的原因。
