造血多能干细胞增殖抑制剂：纯化及其结构测定
Inhibitor of hematopoietic pluripotent stem cell proliferation: purification and determination of its structure.
作者信息
Lenfant M, Wdzieczak-Bakala J, Guittet E, Prome J C, Sotty D, Frindel E
机构信息
Institut de Chimie des Substances Naturelles, Centre National de la Recherche Scientifique, Gif-sur-Yvette, France.
出版信息
Proc Natl Acad Sci U S A. 1989 Feb;86(3):779-82. doi: 10.1073/pnas.86.3.779.
Abstract
We report here a five-step purification procedure that led to the isolation from fetal calf bone marrow extract of a tetrapeptide, Ac-Ser-Asp-Lys-Pro (Mr 487), exerting a high inhibitory activity on the proliferation of hematopoietic pluripotent stem cells [defined here as spleen colony-forming units (CFU-S)]. The structure of this molecule was established from amino acid analysis, fast atom bombardment mass spectrometry, and 1H nuclear magnetic resonance spectral data. This structure was confirmed by comparison with the corresponding synthetic molecule, which presents identical physiochemical characteristics and biological properties. Natural and synthetic peptides administered to mice (at a dose of 100 ng per mouse) after one injection of cytosine arabinonucleoside prevent CFU-S recruitment into DNA synthesis.
摘要
我们在此报告一种五步纯化程序，该程序从胎牛骨髓提取物中分离出一种四肽，即Ac-Ser-Asp-Lys-Pro（分子量487），它对造血多能干细胞的增殖具有高度抑制活性[在此定义为脾集落形成单位（CFU-S）]。通过氨基酸分析、快原子轰击质谱和1H核磁共振光谱数据确定了该分子的结构。通过与相应的合成分子进行比较证实了该结构，合成分子具有相同的物理化学特性和生物学特性。在给小鼠注射一次阿糖胞苷后，给予天然和合成肽（每只小鼠剂量为100 ng）可阻止CFU-S进入DNA合成。
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本文引用的文献
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Effects of CFS-S inhibitors on murine bone marrow during ARA-C treatment--I. Effects on stem cells.CFS-S抑制剂在阿糖胞苷治疗期间对小鼠骨髓的影响——I. 对干细胞的影响
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Inhibitor of stem cell proliferation in normal bone marrow.正常骨髓中干细胞增殖的抑制剂。
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