用四氮唑蓝测定纳摩尔范围内的还原糖。
Determination of reducing sugars in the nanomole range with tetrazolium blue.
作者信息
Jue C K, Lipke P N
出版信息
J Biochem Biophys Methods. 1985 Aug;11(2-3):109-15. doi: 10.1016/0165-022x(85)90046-6.
PMID:4031356
Abstract
Addition of sodium potassium tartrate to basic solutions of tetrazolium blue [(2,2',5,5'-tetraphenyl-3,3'-dimethoxy 4,4'-biphenylene) ditetrazolium chloride] greatly improved their efficacy as colorimetric reagents for reducing sugar determination. This modification increased sensitivity and decreased reaction time. The modified reagent can determine as little as 1 nmol of neutral sugars as well as 2-amino and N-acetyl amino sugars.
摘要
向四氮唑蓝（氯化2,2',5,5'-四苯基-3,3'-二甲氧基-4,4'-联苯撑双四氮唑）的碱性溶液中添加酒石酸钠钾，极大地提高了它们作为比色试剂测定还原糖的效能。这种改进提高了灵敏度并缩短了反应时间。改良后的试剂能够测定低至1纳摩尔的中性糖以及2-氨基糖和N-乙酰氨基糖。
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