对用γ-氨基丁酸（GABA）和别孕烯醇酮功能化的丝素蛋白进行体外和体内评估，以观察其对雪旺细胞和神经元存活的影响。
In vitro and in vivo evaluation of silk fibroin functionalized with GABA and allopregnanolone for Schwann cell and neuron survival.
作者信息
Gennari Chiara Gm, Cilurzo Francesco, Mitro Nico, Caruso Donatella, Minghetti Paola, Magnaghi Valerio
机构信息
Department of Pharmaceutical Sciences, Università degli Studi di Milano, Milan, Italy.
Department of Pharmacological & Biomolecular Sciences, Università degli Studi di Milano, Milan, Italy.
出版信息
Regen Med. 2018 Mar;13(2):141-157. doi: 10.2217/rme-2017-0102. Epub 2017 Nov 21.
AIM
This in vitro and in vivo study reports on silk fibroin (SF) scaffold, functionalized for in situ delivery of GABA and/or allopregnanolone (ALLO), as biomaterial for potential application in tissue engineering and nerve regeneration.
MATERIALS & METHODS: We evaluated the feasibility to design 2D scaffolds (films) made of regenerated Bombyx mori SF, functionalized with GABA and/or ALLO to enhance in vitro biological functions, health, survival and growth of Schwann cells and sensitive neurons of the dorsal root ganglia.
RESULTS & CONCLUSION: Our 2D-SF film showed an efficient loading and controllable release of drugs promoting nerve regeneration. SF functionalized film may be helpful for the development of bioengineered conduits and, in principle, have great potential for long-gap nerve injury repair.
目的
本体内外研究报道了经功能化处理以实现γ-氨基丁酸（GABA）和/或别孕烯醇酮（ALLO）原位递送的丝素蛋白（SF）支架，作为一种生物材料，有望应用于组织工程和神经再生。
材料与方法
我们评估了设计由再生家蚕丝素蛋白制成的二维支架（薄膜）的可行性，该支架经GABA和/或ALLO功能化处理，以增强体外生物学功能、健康状况、雪旺细胞的存活和生长以及背根神经节感觉神经元的生长。
结果与结论
我们的二维丝素蛋白薄膜显示出对促进神经再生药物的高效负载和可控释放。功能化的丝素蛋白薄膜可能有助于生物工程导管的开发，原则上，对长节段神经损伤修复具有巨大潜力。
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