转录组学和激素分析表明，YUC介导的生长素生物合成参与了[植物名称]根茎的芽再生。
Transcriptomic and Hormonal Analyses Reveal that YUC-Mediated Auxin Biogenesis Is Involved in Shoot Regeneration from Rhizome in .
作者信息
Liu Yang, Zhang Hai-Liang, Guo He-Rong, Xie Li, Zeng Rui-Zhen, Zhang Xiang-Qian, Zhang Zhi-Sheng
机构信息
College of Forestry and Landscape Architecture, South China Agricultural University, Guangzhou, China.
出版信息
Front Plant Sci. 2017 Oct 27;8:1866. doi: 10.3389/fpls.2017.01866. eCollection 2017.
, one of the most important orchid genera in horticulture, can be classified into epiphytic and terrestrial species. Generally, epiphytic seedlings can be easily propagated by tissue culture, but terrestrial seedlings are difficult to propagate. To date, the molecular mechanisms underlying the differences in the ease with which terrestrial and epiphytic cymbidiums can be propagated are largely unknown. Using RNA-sequencing, quantitative reverse transcription PCR and enzyme-linked immunosorbent assay, 'Xiaofeng' (CXF), which can be efficiently micropropagated, and terrestrial 'Qijianbaimo' (CSQ), which has a low regeneration ability, were used to explore the molecular mechanisms underlying the micropropagation ability of species. To this end, 447 million clean short reads were generated, and 31,264 annotated unigenes were obtained from 10 cDNA libraries. A total of 1,290 differentially expressed genes (DEGs) were identified between CXF and CSQ during shoot induction. Gene ontology (GO) enrichment analysis indicated that the DEGs were significantly enriched in auxin pathway-related GO terms. Further analysis demonstrated that YUC and GH3 family genes, which play crucial roles in the regulation of auxin/IAA (indole-3-acetic acid) metabolism, acted quickly in response to shoot induction culture and were closely correlated with variation in shoot regeneration between CXF and CSQ. In addition, the study showed that IAA accumulated rapidly and significantly during shoot induction in CXF compared to that in CSQ; in contrast, no significant changes in other hormones were observed between CXF and CSQ. Furthermore, shoot regeneration in CXF was inhibited by a yucasin-auxin biosynthesis inhibitor, indicating that increased IAA level is required for high-frequency shoot regeneration in CXF. In conclusion, our study revealed that YUC-mediated auxin biogenesis is involved in shoot regeneration from rhizome in
作为园艺学中最重要的兰花属之一，可分为附生种和地生种。一般来说，附生幼苗可通过组织培养轻松繁殖，而地生幼苗则难以繁殖。迄今为止，地生和附生大花蕙兰在繁殖难易程度上存在差异的分子机制在很大程度上尚不清楚。利用RNA测序、定量逆转录PCR和酶联免疫吸附测定，以可高效微繁殖的“小凤”（CXF）和再生能力低的地生“奇剑白墨”（CSQ）为材料，探讨大花蕙兰微繁殖能力的分子机制。为此，共产生了4.47亿条干净的短读段，并从10个cDNA文库中获得了31264个注释单基因。在芽诱导过程中，共鉴定出1290个差异表达基因（DEG）。基因本体（GO）富集分析表明，这些DEG在生长素途径相关的GO术语中显著富集。进一步分析表明，在生长素/吲哚-3-乙酸（IAA）代谢调控中起关键作用的YUC和GH3家族基因，对芽诱导培养反应迅速，且与CXF和CSQ之间的芽再生变异密切相关。此外，研究表明，与CSQ相比，CXF在芽诱导过程中IAA迅速且显著积累；相反，CXF和CSQ之间其他激素未观察到显著变化。此外，CXF的芽再生受到yucasin-生长素生物合成抑制剂的抑制，表明CXF高频芽再生需要增加IAA水平。总之，我们的研究表明，YUC介导的生长素生物合成参与了根状茎的芽再生。
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