Chang C, Chang W C
Institute of Botany, Academia Sinica, Taipei, Taiwan 115, Republic of China Fax no.: +886-2-7827954 E-mail:
Plant Cell Rep. 1998 Feb;17(4):251-255. doi: 10.1007/s002990050387.
Totipotent calli of Cymbidium ensifolium var. misericors, a locally grown orchid of high commercial value, were induced from sections of pseudobulbs, rhizomes and roots of seed-derived plantlets on 1/2-strength Murashige and Skoog medium plus 10 mg/l 2,4-dichlorophenoxyacetic acid and 0.1 mg/l thiadiazuron. The calli could be maintained by subculturing in the same medium. The calli could be induced to develop further along one of three distinct morphogeneic routes: (1) production of rhizomes, (2) production of shoot buds, or (3) development of granular embryoids. Efficient mass propagation was possible via rhizome proliferation and embryoid formation.
寒兰（Cymbidium ensifolium var. misericors）是一种具有高商业价值的本地种植兰花，其全能愈伤组织是从种子衍生苗的假鳞茎、根状茎和根段在1/2强度的Murashige和Skoog培养基加10毫克/升2,4 - 二氯苯氧乙酸和0.1毫克/升噻苯隆上诱导产生的。这些愈伤组织可通过在相同培养基中继代培养来维持。愈伤组织能够沿着三种不同的形态发生途径之一进一步发育：（1）根状茎的产生，（2）芽的产生，或（3）颗粒状胚状体的发育。通过根状茎增殖和胚状体形成可实现高效的大规模繁殖。
