Fazel A R, Sumida H, Schulte B A, Thompson R P
Department of Anatomy and Cell Biology, Medical University of South Carolina, Charleston 29425.
Am J Anat. 1989 Jan;184(1):76-84. doi: 10.1002/aja.1001840109.
Glycoconjugates, particularly their sugar side chains, play important roles in embryonic development. Changes in cell-surface-associated glycoconjugates are known to affect cell differentiation, cellular interactions, and other developmental phenomena during embryogenesis. The embryonic heart goes through a series of complicated morphologic events during development. Of particular interest is morphogenesis of the outflow tract. This region of the embryonic heart originates from more than one cell population and undergoes a complex process of septation during formation of the great vessels. Histochemical analysis with a series of fucose-specific lectins conjugated to horseradish peroxidase has revealed the presence of a fucosylated glycoconjugate in the outflow tract of the developing heart. The results reveal further that the expression of the fucosylated glycoconjugate is stage-dependent and thus probably genetically regulated. The timing and distribution of staining with the lectin OFA suggest that this fucosylated glycoconjugate may play a role in directing the migration of neural crest cells into the heart and subsequent formation of the conus septum.
糖缀合物，尤其是其糖侧链，在胚胎发育中发挥着重要作用。已知细胞表面相关糖缀合物的变化会影响胚胎发生过程中的细胞分化、细胞间相互作用以及其他发育现象。胚胎心脏在发育过程中经历一系列复杂的形态学事件。特别值得关注的是流出道的形态发生。胚胎心脏的这个区域起源于多个细胞群体，并且在大血管形成过程中经历复杂的分隔过程。用一系列与辣根过氧化物酶偶联的岩藻糖特异性凝集素进行组织化学分析，揭示了发育中心脏流出道中存在一种岩藻糖基化糖缀合物。结果进一步表明，岩藻糖基化糖缀合物的表达是阶段依赖性的，因此可能受基因调控。用凝集素OFA染色的时间和分布表明，这种岩藻糖基化糖缀合物可能在引导神经嵴细胞迁移到心脏以及随后的圆锥隔形成中发挥作用。
