Jankowski M, Spużak J, Kubiak K, Glińska-Suchocka K, Biernat M
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Pol J Vet Sci. 2017 Sep 26;20(3):491-499. doi: 10.1515/pjvs-2017-0059.
The aim of this study was to assess the suitability of invasive and non-invasive methods used to diagnose Helicobacter spp. in the stomachs of dogs. The study was carried out on 30 dogs of both sexes and different breeds, between one and 15 years old. A histopathologic examination, a microbiological culture, a rapid urease test, a direct bacteriological preparation and a nested PCR assay were carried out. Gastric Helicobacter spp. was identified in gastric biopsy specimens from 16 (53.3%) dogs using direct bacteriological preparation, in four (13.3%) dogs based on a culture, in 23 (76.6%) dogs using the rapid urease test and in 21 (70,0%) dogs based on a histopathological assessment of the biopsy specimens. The nested PCR of the gastric biopsy specimens revealed gastric Helicobacter spp. in all the dogs (100%). A saliva PCR assay revealed gastric Helicobacter spp. in 23 (76.6%) dogs, while stool PCR revealed the bacterium in seven (23.3%) dogs. We found that invasive methods were more accurate than non-invasive methods in detecting a Helicobacter spp. infection in dogs. In addition, the nested PCR method used to evaluate the gastric mucosal biopsy specimens was the most accurate test for detecting Helicobacter spp. It was further found that the PCR-based saliva assay was the best non-invasive method for detecting Helicobacter spp. However, taking into consideration that most of the diagnostic methods used to detect this bacterium have drawbacks, at least two diagnostic methods should be used to detect Helicobacter spp. as is done in human medicine.
本研究的目的是评估用于诊断犬胃内幽门螺杆菌属的侵入性和非侵入性方法的适用性。该研究对30只年龄在1至15岁之间、不同品种的雌雄犬进行。进行了组织病理学检查、微生物培养、快速尿素酶试验、直接细菌学制片和巢式聚合酶链反应（PCR）检测。通过直接细菌学制片在16只（53.3%）犬的胃活检标本中鉴定出胃幽门螺杆菌属，基于培养在4只（13.3%）犬中鉴定出，通过快速尿素酶试验在23只（76.6%）犬中鉴定出，基于活检标本的组织病理学评估在21只（70.0%）犬中鉴定出。胃活检标本的巢式PCR检测显示所有犬（100%）均有胃幽门螺杆菌属。唾液PCR检测在23只（76.6%）犬中检测到胃幽门螺杆菌属，而粪便PCR检测在7只（
