Recordati Camilla, Gualdi Valentina, Tosi Sabrina, Facchini Roberto Vailati, Pengo Graziano, Luini Mario, Simpson Kenneth W, Scanziani Eugenio
Dipartimento di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Sezione di Anatomia Patologica Veterinaria e Patologia Aviare, Facoltà di Medicina Veterinaria, Università degli Studi di Milano, Milano, Italy.
Vet Microbiol. 2007 Jan 31;119(2-4):346-51. doi: 10.1016/j.vetmic.2006.08.029. Epub 2006 Sep 8.
The mode of acquisition of gastric Helicobacter spp. infection in dogs has not been determined. It is suspected that oral-oral and faecal-oral transmission may be involved. The present study sought to determine if Helicobacter spp. DNA is present in the oral cavity of healthy and vomiting dogs. Thirty-eight pet dogs (27 vomiting and 11 clinically healthy) were studied. The presence of Helicobacter spp. was determined by single and nested PCR evaluation of DNA extracted from saliva, dental plaque and gastric biopsy samples. Helicobacter spp. DNA was detected by nested PCR in 36 (94.7%) gastric biopsies, 17 (44.7%) dental plaque and 19 (50%) saliva samples out of the 38 dogs examined. Overall 27 (71.1%) dogs screened by nested PCR were found to harbour Helicobacter spp. DNA in the oral cavity (dental plaque and/or saliva). There was no significant difference in the prevalence of Helicobacter spp. DNA in the oral cavity of vomiting and healthy dogs, and the time from vomiting to oral sampling did not have significant impact. This study confirms the high prevalence of gastric Helicobacter spp. infection in dogs, and reveals that Helicobacter spp. DNA is detectable in the oral cavity of over 70% of dogs. These findings support the possibility of oral-oral transmission between dogs and that the canine oral cavity may act as source of non-pylori Helicobacter spp. infection for humans.
犬胃内幽门螺杆菌属感染的获取方式尚未确定。怀疑可能涉及口口传播和粪口传播。本研究旨在确定健康犬和呕吐犬的口腔中是否存在幽门螺杆菌属DNA。研究了38只宠物犬（27只呕吐犬和11只临床健康犬）。通过对从唾液、牙菌斑和胃活检样本中提取的DNA进行单重和巢式PCR评估来确定幽门螺杆菌属的存在。在38只受试犬中，通过巢式PCR在36份（94.7%）胃活检样本、17份（44.7%）牙菌斑样本和19份（50%）唾液样本中检测到幽门螺杆菌属DNA。总体而言，通过巢式PCR筛查的27只（71.1%）犬被发现口腔（牙菌斑和/或唾液）中含有幽门螺杆菌属DNA。呕吐犬和健康犬口腔中幽门螺杆菌属DNA的患病率没有显著差异，从呕吐到口腔采样的时间也没有显著影响。本研究证实了犬胃内幽门螺杆菌属感染的高患病率，并揭示超过70%的犬口腔中可检测到幽门螺杆菌属DNA。这些发现支持了犬之间口口传播的可能性，以及犬口腔可能作为人类非幽门螺杆菌属感染源的可能性。
