Pejin Boris, Tešanović Kristina, Jakovljević Dragica, Kaišarević Sonja, Šibul Filip, Rašeta Milena, Karaman Maja
a Department of Life Sciences, Institute for Multidisciplinary Research - IMSI , University of Belgrade , Belgrade , Serbia.
b Faculty of Sciences, Department of Biology and Ecology , University of Novi Sad , Novi Sad , Serbia.
Nat Prod Res. 2019 Mar;33(5):750-754. doi: 10.1080/14786419.2017.1405417. Epub 2017 Nov 22.
The polysaccharide (PSH) extracts from the edible mushroom species Coprinus comatus and Coprinellus truncorum were screened in liquid for their acetylcholinesterase inhibitory (AChE) activity. Both extracts were found to display inhibition of the aforementioned enzyme reaching similar IC values of 0.62 ± 0.07 and 0.61 ± 0.03 mg/mL, respectively. According to the means of FTIR spectroscopy, these PSH extracts mostly contained β-glucans. However, the presence of some proteins and polyphenolics as minor ingredients were also detected. Compared with existing literature data for anti-AChE activity of the sugar samples, the findings within this study may be treated as a profound bioactivity. Consequently, this study puts some light on the possible use of the screened macrofungi in the palliative treatment of Alzheimer's disease.
对毛头鬼伞和截形小脆柄菇这两种食用菌的多糖（PSH）提取物进行了液体乙酰胆碱酯酶抑制（AChE）活性筛选。发现两种提取物均对上述酶有抑制作用，其IC值分别达到相似的0.62±0.07和0.61±0.03mg/mL。根据傅里叶变换红外光谱分析方法，这些PSH提取物主要含有β-葡聚糖。然而，也检测到一些蛋白质和多酚类物质作为次要成分存在。与现有文献中糖类样品的抗AChE活性数据相比，本研究结果可被视为具有显著的生物活性。因此，本研究为筛选出的大型真菌在阿尔茨海默病姑息治疗中的可能应用提供了一些线索。
