Institute of Physiology and Biochemistry, Faculty of Biology, University of Belgrade, Belgrade, Serbia.
Department of Chemistry, Institute of Chemistry, Technology and Metallurgy - ICTM, University of Belgrade, Belgrade, Serbia.
Nat Prod Res. 2023 Jun;37(11):1838-1843. doi: 10.1080/14786419.2022.2118743. Epub 2022 Sep 2.
Fungi are an important source of polysaccharides (PSH) and phenolic compounds (PC). Numerous studies have highlighted the beneficial effects of fungal consumption, but the impact of submerged cultivated mycelia (M) and filtrate (F) has not been fully investigated. We aimed to investigate the cytotoxic activity of isolated crude PSH and exopolysaccharides (ePSH) of submerged cultivated M and F of edible and species. Both PSH and ePSH exhibited significant cytotoxic activity towards HepG2 cancer cells of human origin (three-way ANOVA). The PSH/ePSH was more efficient inducing maximal reduction in cell viability (≈50% at 450 µg/mL) after 24 h while PSH/ePSH needed 72 h to reach similar effect (≈60% at 450 µg/mL). Partial least square regression (PLSR) analysis indicated that specific phenolic composition of the PSH/ePSH could be responsible for the difference in their activity.
真菌是多糖 (PSH) 和酚类化合物 (PC) 的重要来源。大量研究强调了真菌消费的有益作用，但对深层培养菌丝体 (M) 和滤液 (F) 的影响尚未进行全面研究。我们旨在研究分离的粗 PSH 和深层培养 M 和 F 的胞外多糖 (ePSH) 的细胞毒性活性，这些 M 和 F 来自可食用的 和 物种。PSH 和 ePSH 均对人源 HepG2 癌细胞表现出显著的细胞毒性活性 (三因素方差分析)。在 24 小时内， PSH/ePSH 更有效地诱导细胞活力最大降低 (≈450 μg/mL 时约 50%)，而 PSH/ePSH 需要 72 小时才能达到类似的效果 (≈450 μg/mL 时约 60%)。偏最小二乘回归 (PLSR) 分析表明，PSH/ePSH 的特定酚类组成可能是其活性差异的原因。
