Institute of Chemistry, The Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem , Jerusalem 91904, Israel.
Nano Lett. 2017 Mar 8;17(3):2043-2048. doi: 10.1021/acs.nanolett.7b00093. Epub 2017 Feb 14.
Cu-ion-modified graphene oxide nanoparticles, Cu-GO NPs, act as a heterogeneous catalyst mimicking functions of horseradish peroxidase, HRP, and of NADH peroxidase. The Cu-GO NPs catalyze the oxidation of dopamine to aminochrome by HO and catalyze the generation of chemiluminescence in the presence of luminol and HO. The Cu-GO NPs provide an active material for the chemiluminescence detection of HO and allow the probing of the activity of HO-generating oxidases and the detection of their substrates. This is exemplified with detecting glucose by the aerobic oxidation of glucose by glucose oxidase and the Cu-GO NP-stimulated chemiluminescence intensity generated by the HO product. Similarly, the Cu-GO NPs catalyze the HO oxidation of NADH to the biologically active NAD cofactor. This catalytic system allows its conjugation to biocatalytic transformations involving NAD-dependent enzyme, as exemplified for the alcohol dehydrogenase-catalyzed oxidation of benzyl alcohol to benzoic acid through the Cu-GO NPs-catalyzed regeneration of NAD.
铜离子修饰的氧化石墨烯纳米粒子（Cu-GO NPs）作为一种非均相催化剂，模拟辣根过氧化物酶（HRP）和烟酰胺腺嘌呤二核苷酸过氧化物酶（NADH 过氧化物酶）的功能。Cu-GO NPs 可以催化 HO 氧化多巴胺生成氨基胆色素，并在鲁米诺和 HO 的存在下催化化学发光的产生。Cu-GO NPs 为 HO 的化学发光检测提供了一种活性材料，并允许探测 HO 生成氧化酶的活性及其底物的检测。这可以通过葡萄糖氧化酶有氧氧化葡萄糖和 Cu-GO NP 刺激 HO 产物产生的化学发光强度来检测葡萄糖为例进行说明。同样，Cu-GO NPs 可以催化 HO 氧化 NADH 生成具有生物活性的 NAD 辅助因子。该催化体系允许其与涉及 NAD 依赖酶的生物催化转化结合，如通过 Cu-GO NPs 催化的 NAD 再生来实现醇脱氢酶催化的苯甲醇氧化生成苯甲酸的例子。
