Xu Kongliang, Chen Xuexiao, Zheng Renchao, Zheng Yuguo
Key Laboratory of Bioorganic Synthesis of Zhejiang Province, College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou, China.
Engineering Research Center of Bioconversion and Biopurification of Ministry of Education, Zhejiang University of Technology, Hangzhou, China.
Front Bioeng Biotechnol. 2020 Jun 30;8:660. doi: 10.3389/fbioe.2020.00660. eCollection 2020.
Multi-enzyme biocatalysis is an important technology to produce many valuable chemicals in the industry. Different strategies for the construction of multi-enzyme systems have been reported. In particular, immobilization of multi-enzymes on the support materials has been proved to be one of the most efficient approaches, which can increase the enzymatic activity via substrate channeling and improve the stability and reusability of enzymes. A general overview of the characteristics of support materials and their corresponding attachment techniques used for multi-enzyme immobilization will be provided here. This review will focus on the materials-based techniques for multi-enzyme immobilization, which aims to present the recent advances and future prospects in the area of multi-enzyme biocatalysis based on support immobilization.
多酶生物催化是工业生产许多有价值化学品的一项重要技术。已经报道了构建多酶系统的不同策略。特别是，将多酶固定在载体材料上已被证明是最有效的方法之一，它可以通过底物通道化提高酶活性，并改善酶的稳定性和可重复使用性。本文将概述用于多酶固定化的载体材料的特性及其相应的固定技术。本综述将重点关注基于材料的多酶固定化技术，旨在介绍基于载体固定化的多酶生物催化领域的最新进展和未来前景。
