Muñiz Alethia, Garcia Abraham H, Pérez Rosa M, García Efren V, González Daphne E
CONACYT-IPICYT/CIIDZA, Camino a la Presa de San José 2055, Col. Lomas 4 sección, CP 78216, San Luis Potosí, México.
Laboratorio de Investigación de Productos Naturales, Escuela Superior de Ingeniería Química e Industrias Extractivas, Av. Instituto Politécnico Nacional s/n, Unidad Profesional Adolfo Lopez Mateos, Col. Zacatenco, CP 07758, Mexico City, México.
Diabetes Ther. 2018 Feb;9(1):67-74. doi: 10.1007/s13300-017-0335-7. Epub 2017 Nov 22.
Hyperglycemia plays an important role in the pathogenesis of diabetic complications, as it increases protein glycation, as well as the progressive accumulation of advanced glycation end products (AGEs), which are complex structures that produce fluorescence. The glycation reaction raises the levels of protein carbonyl, N -(carboxymethyl)lysine (CML), and fructosamine and decreases the level of thiol groups.
In the present study, the antiglycation activity was determined by fluorescence intensity using the bovine serum albumin (BSA)/glucose, CML method, and the level of fructosamine. The oxidation of proteins was determined by the carbonyl protein content and thiol groups.
The results show that the hexane extract of Acca sellowiana (FOH) at different concentrations (0.30-5 mg/ml) significantly inhibited the formation of AGEs in the BSA/glucose model during the 4 weeks of the study. FOH reduced the levels of fructosamine and CML. Our results showed a significant effect of FOH in the prevention of oxidative damage of proteins, as well as an effect on the oxidation of thiol groups and carbonyl proteins.
The present study indicates that FOH is effective in inhibiting the glycation of proteins in vitro, so it can prevent or ameliorate the chronic conditions of diabetes associated with the formation of AGEs.
高血糖在糖尿病并发症的发病机制中起重要作用，因为它会增加蛋白质糖基化以及晚期糖基化终产物（AGEs）的逐步积累，AGEs是产生荧光的复杂结构。糖基化反应会提高蛋白质羰基、N -（羧甲基）赖氨酸（CML）和果糖胺的水平，并降低巯基水平。
在本研究中，使用牛血清白蛋白（BSA）/葡萄糖、CML方法通过荧光强度测定抗糖基化活性以及果糖胺水平。通过羰基蛋白含量和巯基来测定蛋白质的氧化。
结果表明，在为期4周的研究中，不同浓度（0.30 - 5毫克/毫升）的费约果己烷提取物（FOH）显著抑制了BSA/葡萄糖模型中AGEs的形成。FOH降低了果糖胺和CML的水平。我们的结果表明，FOH在预防蛋白质氧化损伤方面具有显著作用，并且对巯基和羰基蛋白的氧化也有影响。
本研究表明，FOH在体外有效抑制蛋白质糖基化，因此它可以预防或改善与AGEs形成相关的糖尿病慢性病症。
