Program in Clinical Biochemistry and Molecular Medicine, Department of Clinical Chemistry, Faculty of Allied Health Sciences, Chulalongkorn University, Bangkok 10330, Thailand.
Molecules. 2013 May 30;18(6):6439-54. doi: 10.3390/molecules18066439.
The inhibitory activity of isoferulic acid (IFA) on fructose- and glucose-mediated protein glycation and oxidation of bovine serum albumin (BSA) was investigated. Our data showed that IFA (1.25-5 mM) inhibited the formation of fluorescent advanced glycation end products (AGEs) and non-fluorescent AGE [Nε-(carboxymethyl) lysine: CML], as well as the level of fructosamine. IFA also prevented protein oxidation of BSA indicated by decreasing protein carbonyl formation and protein thiol modification. Furthermore, IFA suppressed the formation of β-cross amyloid structures of BSA. Therefore, IFA might be a new promising anti-glycation agent for the prevention of diabetic complications via inhibition of AGEs formation and oxidation-dependent protein damage.
异阿魏酸（IFA）对果糖和葡萄糖介导的牛血清白蛋白（BSA）糖化和氧化的抑制活性进行了研究。我们的数据表明，IFA（1.25-5 mM）抑制了荧光晚期糖基化终产物（AGEs）和非荧光 AGE[Nε-（羧甲基）赖氨酸：CML]的形成，以及果糖胺的水平。IFA 还通过减少蛋白质羰基形成和蛋白质巯基修饰来防止 BSA 的蛋白质氧化。此外，IFA 抑制了 BSA 的β-交叉淀粉样结构的形成。因此，IFA 可能是一种新的有前途的抗糖化剂，可通过抑制 AGEs 的形成和氧化依赖性蛋白质损伤来预防糖尿病并发症。
