Tomita N, Izumoto Y, Horii A, Doi S, Yokouchi H, Ogawa M, Mori T, Matsubara K
Institute for Molecular and Cellular Biology, Osaka University, Suita, Japan.
Biochem Biophys Res Commun. 1989 Jan 31;158(2):569-75. doi: 10.1016/s0006-291x(89)80087-7.
The cDNA clone encoding human prechymotrypsinogen was isolated from a human pancreas cDNA library and its nucleotide sequence was determined. The sequence consists of a 16 bp 5' non-coding region, a 789 bp amino acid coding region and a 60 bp 3' non-coding region. The predicted product consists of 263 amino acids, including 18 amino acids for a signal peptide and 15 amino acids possible for an activation peptide. Southern blot analyses using the cloned cDNA as a probe revealed that human genomic DNA carries at least two genes that are related to chymotrypsinogen.
从人胰腺cDNA文库中分离出编码人胰凝乳蛋白酶原前体的cDNA克隆，并测定了其核苷酸序列。该序列由一个16bp的5'非编码区、一个789bp的氨基酸编码区和一个60bp的3'非编码区组成。预测产物由263个氨基酸组成，包括一个18个氨基酸的信号肽和15个可能的激活肽氨基酸。用克隆的cDNA作为探针进行Southern印迹分析表明，人基因组DNA携带至少两个与胰凝乳蛋白酶原相关的基因。
