Browne R M, Tyas M J
J Oral Rehabil. 1979 Oct;6(4):365-74. doi: 10.1111/j.1365-2842.1979.tb00417.x.
In vitro methods for the biological evaluation of dental restorative materials are discussed, with particular reference to the type of cells used, the physical state of the test material, the method of establishing cell-material contact, and the criteria of cytotoxicity. It is postulated that in vitro methods should provide a reliable assessment of the toxicity of dental restorative materials, but since the current in vitro methods do not mimic in vivo conditions sufficiently closely, the correlation between in vitro and in vivo results is at present poor. The development of in vitro methods more closely simulating in vivo circumstances is advocated.
讨论了牙科修复材料生物学评价的体外方法，特别提及所使用的细胞类型、测试材料的物理状态、建立细胞与材料接触的方法以及细胞毒性标准。据推测，体外方法应能可靠地评估牙科修复材料的毒性，但由于目前的体外方法与体内条件的模拟不够紧密，体外和体内结果之间目前的相关性较差。提倡开发更紧密模拟体内情况的体外方法。
