Matzku S, Wenzel A, Liu S, Zöller M
Institute of Radiology and Pathophysiology, German Cancer Research Center, Heidelberg.
Cancer Res. 1989 Mar 1;49(5):1294-9.
Variants AS and ASML of the BSp73 rat tumor differ markedly with respect to morphology and to the capacity for spontaneous metastasis via the lymphatics. Monoclonal antibodies (MAbs) were raised in order to identify surface molecules associated with both phenotypes. Mice were immunized with either whole cells or isolated membranes and hybridoma supernatants were screened according to the criteria of selective binding to cultured cells of the variant used for immunization. Of two MAbs reacting with the nonmetastasizing AS variant, one showed immunostaining of AS tissue. From 11 MAbs binding to the metastatic ASML variant, 6 showed specific staining of ASML tissue, while the remaining 5 MAbs showed cross-reaction with normal rat tissues. According to Western blot data, the 6 MAbs selectively binding to ASML tissue identified at least 2 different antigens, one of them showing up as a complex of 12 bands.
BSp73大鼠肿瘤的AS和ASML变体在形态以及通过淋巴管发生自发转移的能力方面存在显著差异。为了鉴定与这两种表型相关的表面分子，制备了单克隆抗体（MAb）。用全细胞或分离的膜对小鼠进行免疫，并根据与用于免疫的变体的培养细胞的选择性结合标准筛选杂交瘤上清液。在与非转移性AS变体反应的两种MAb中，有一种显示出对AS组织的免疫染色。在与转移性ASML变体结合的11种MAb中，6种显示出对ASML组织的特异性染色，而其余5种MAb与正常大鼠组织发生交叉反应。根据蛋白质印迹数据，选择性结合ASML组织的6种MAb鉴定出至少2种不同的抗原，其中一种表现为12条带的复合物。
