Department of Pathology and Pathophysiology, School of Medicine and Life Sciences, Nanjing University of Traditional Chinese Medicine, Nanjing, 210023 Jiangsu Province, China.
Centre of Reproductive Medicine, Jinling Hospital, Nanjing University School of Medicine, Nanjing, 210002 Jiangsu Province, China.
Reprod Biomed Online. 2018 Jan;36(1):20-25. doi: 10.1016/j.rbmo.2017.09.003. Epub 2017 Sep 21.
The accumulation of advanced glycation end products (AGE) is associated with ovarian dysfunction. This study examines whether the accumulation of AGE in follicular fluid affects ovarian responsiveness and embryo quality during IVF/intracytoplasmic sperm injection (IVF/ICSI) with a gonadotrophin-releasing hormone (GnRH) agonist protocol. The levels of AGE in follicular fluid were measured in 127 women undergoing IVF/ICSI in GnRH agonist cycles. Plasma hormones were also measured. Embryos were graded using standard approaches. There were inverse associations between follicular fluid AGE concentration and number of oocytes retrieved, number of fertilized oocytes, number of high-quality embryos, fertilization rate and high-quality embryo rate, adjusted for potential confounders. AGE concentration in follicular fluid was significantly higher in women with an ovarian response below the target (<7 oocytes) compared with those reaching the target (7-15 oocytes) or above the target (>15 oocytes). The cut-off value of 15.3 µg/ml for follicular fluid AGE showed 84.6% sensitivity and 55.5% specificity in evaluating the response to ovarian stimulation as below the target. The results suggest that ovarian responsiveness and embryo quality are related to intraovarian exposure to AGE.
糖基化终产物（AGE）的积累与卵巢功能障碍有关。本研究通过使用促性腺激素释放激素（GnRH）激动剂方案的体外受精/卵胞浆内单精子注射（IVF/ICSI），检查卵泡液中 AGE 的积累是否会影响卵巢反应性和胚胎质量。在 GnRH 激动剂周期中，对 127 名接受 IVF/ICSI 的女性的卵泡液中的 AGE 水平进行了测量。还测量了血浆激素。胚胎采用标准方法进行分级。卵泡液 AGE 浓度与获得的卵母细胞数量、受精卵数量、优质胚胎数量、受精率和优质胚胎率呈负相关，经潜在混杂因素调整后。与达到目标（7-15 个卵母细胞）或超过目标（>15 个卵母细胞）的女性相比，卵巢反应低于目标（<7 个卵母细胞）的女性卵泡液中的 AGE 浓度显着更高。卵泡液 AGE 的截断值为 15.3µg/ml，在评估卵巢刺激反应低于目标时，其敏感性为 84.6%，特异性为 55.5%。结果表明，卵巢反应性和胚胎质量与卵巢内暴露于 AGE 有关。
