Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Institute of Environment and Sustainable Development in Agriculture, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
Bioresour Technol. 2018 Feb;250:247-255. doi: 10.1016/j.biortech.2017.11.015. Epub 2017 Nov 7.
Anaerobic digestion (AD) has been applied to animal manure stabilization, and antibiotics is frequently found in animal manure. However, antibiotic degradation and microbial community structures during two-stage AD (acidification and methanogenesis) remain poorly understood. This experiments on two-stage anaerobic swine manure digesters were performed to investigate the degradation mechanisms and effects of chlortetracycline (CTC) and oxytetracycline (OTC) on microbial community structures. Results showed that acidification and methanogenesis showed good degradation performance for manure containing CTC and OTC at 60 and 40 mg/kg·TS, respectively. CTC and OTC were degraded by 59.8% and 41.3% in the acidogenic stage and by 76.3% and 78.3% in the methanogenic stage, respectively. CTC and OTC negatively affected bacterial community in methanogenic and acidogenic stages, respectively. They also adversely influenced the archaeal species in the methanogenic stage. Two-stage AD was proposed to treat manure containing antibiotics and to reduce the negative effects of antibiotics on AD.
厌氧消化（AD）已应用于动物粪便稳定化，而抗生素经常存在于动物粪便中。然而，在两段式 AD（酸化和产甲烷）过程中，抗生素的降解和微生物群落结构仍知之甚少。本实验采用两段式厌氧猪粪消化器，研究了氯四环素（CTC）和土霉素（OTC）对微生物群落结构的降解机制和影响。结果表明，酸化和产甲烷阶段对含 CTC 和 OTC 的粪便分别在 60 和 40 mg/kg·TS 时表现出良好的降解性能。CTC 和 OTC 在产酸阶段分别降解了 59.8%和 41.3%，在产甲烷阶段分别降解了 76.3%和 78.3%。CTC 和 OTC 分别对产甲烷和产酸阶段的细菌群落产生负面影响。它们还对产甲烷阶段的古菌种类产生不利影响。两段式 AD 被提议用于处理含抗生素的粪便，并减少抗生素对 AD 的负面影响。
