State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.
State Key Laboratory of Environmental Aquatic Chemistry, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.
J Environ Sci (China). 2018 May;67:206-215. doi: 10.1016/j.jes.2017.08.023. Epub 2017 Sep 8.
Swine manure was typical for the combined pollution of heavy metals and antibiotics. The effects of widely used veterinary antibiotic chlortetracycline (CTC), Cu and their combination on swine manure anaerobic digestion performance and microbial community have never been investigated. Thus, four 2L anaerobic digestion reactors were established including reactor A (control), B (CTC spiked by 0.5g/kg dry weight, dw), C (Cu spiked by 5g/kg dw) and D (combination of CTC, 0.5g/kg dw, and Cu, 5g/kg dw), and dynamics of bacterial and archaeal community structure was investigated using high throughput sequencing method. Results showed that addition of CTC and Cu separately could increase the total biogas production by 21.6% and 15.8%, respectively, while combination of CTC and Cu severely inhibited anaerobic digestion (by 30.3%). Furthermore, corresponding to different stages and reactors, four kinds of microbes including bacteria and archaea were described in detail, and the effects of CTC, Cu and their combination mainly occurred at hydrolysis and acidification phases. The addition of Cu alone changed the dynamics of archaeal community significantly. It was genus Methanomassiliicoccus that dominated at the active methane production for A, B and D, while it was genus Methanobrevibacter and Methanoculleus for C.
猪粪是重金属和抗生素联合污染的典型代表。广泛应用的兽用抗生素金霉素（CTC）、Cu 及其组合对猪粪厌氧消化性能和微生物群落的影响尚未被研究过。因此，建立了四个 2L 厌氧消化反应器，包括反应器 A（对照）、B（以 0.5g/kg 干重（dw）的浓度添加 CTC）、C（以 5g/kg dw 的浓度添加 Cu）和 D（以 0.5g/kg dw 的浓度添加 CTC 和 5g/kg dw 的浓度添加 Cu），并采用高通量测序方法研究了细菌和古菌群落结构的动态变化。结果表明，单独添加 CTC 和 Cu 分别可以使总沼气产量增加 21.6%和 15.8%，而 CTC 和 Cu 的组合则严重抑制了厌氧消化（减少了 30.3%）。此外，对应于不同的阶段和反应器，详细描述了包括细菌和古菌在内的四种微生物，CTC、Cu 及其组合的影响主要发生在水解和酸化阶段。单独添加 Cu 会显著改变古菌群落的动态。在 A、B 和 D 中，主导活性甲烷生产的是甲烷甲烷球菌属（Methanomassiliicoccus），而在 C 中则是甲烷短杆菌属（Methanobrevibacter）和甲烷微菌属（Methanoculleus）。
