Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia.
Department of Chemical and Environmental Engineering, Faculty of Engineering, University of Nottingham Malaysia Campus, Jalan Broga, 43500 Semenyih, Selangor Darul Ehsan, Malaysia.
Bioresour Technol. 2018 Feb;250:306-316. doi: 10.1016/j.biortech.2017.11.050. Epub 2017 Nov 21.
Liquid Biphasic Flotation (LBF) is an advanced recovery method that has been effectively applied for biomolecules extraction. The objective of this investigation is to incorporate the fermentation and extraction process of lipase from Burkholderia cepacia using flotation system. Initial study was conducted to compare the performance of bacteria growth and lipase production using flotation and shaker system. From the results obtained, bacteria shows quicker growth and high lipase yield via flotation system. Integration process for lipase separation was investigated and the result showed high efficiency reaching 92.29% and yield of 95.73%. Upscaling of the flotation system exhibited consistent result with the lab-scale which are 89.53% efficiency and 93.82% yield. The combination of upstream and downstream processes in a single system enables the acceleration of product formation, improves the product yield and facilitates downstream processing. This integration system demonstrated its potential for biomolecules fermentation and separation that possibly open new opportunities for industrial production.
液相双相浮选 (LBF) 是一种先进的回收方法，已被有效地应用于生物分子的提取。本研究的目的是将发酵和提取 Burkholderia cepacia 脂肪酶的过程与浮选系统相结合。初步研究比较了浮选和摇床系统对细菌生长和脂肪酶生产的性能。从获得的结果来看，细菌通过浮选系统表现出更快的生长和更高的脂肪酶产量。还研究了脂肪酶分离的集成工艺，结果表明效率高达 92.29%，收率为 95.73%。浮选系统的放大实验与实验室规模实验结果一致，效率为 89.53%，收率为 93.82%。在单个系统中整合上游和下游工艺可以加速产物形成，提高产物收率并促进下游加工。该集成系统展示了其在生物分子发酵和分离方面的潜力，为工业生产开辟了新的机会。
