Leong Hui Yi, Chang Yu-Kaung, Ooi Chien Wei, Law Chung Lim, Julkifle Advina Lizah, Show Pau Loke
Bioseparation Research Group, Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, Semenyih, Malaysia.
Department of Chemical Engineering, Graduate School of Biochemical Engineering, Ming Chi University of Technology, Taipei, Taiwan.
Front Chem. 2019 Apr 3;7:201. doi: 10.3389/fchem.2019.00201. eCollection 2019.
Nowadays, downstream bioprocessing industries inclines towards the development of a green and high efficient bioseparation technology. Betacyanins are presently gaining higher interest in the food science as driven by their high tinctorial strength and health promoting functional properties. In this study, a novel green integration process of liquid biphasic electric partitioning system (LBEPS) was proposed for betacyanins extraction from peel and flesh of red-purple pitaya. Initially, the betacyanins extraction using LBEPS with initial settings was compared with that of liquid biphasic partitioning system (LBPS), and the results revealed that both systems demonstrated a comparable betacyanins extraction. This was followed by further optimizing the LBEPS for better betacyanins extraction. Several operating parameters including operation time, voltage applied, and position of graphitic electrodes in the system were investigated. Moreover, comparison between optimized LBEPS and LBPS with optimized conditions of electric system (as post-treatment) as well as color characterization and antioxidant properties assessment were conducted. Overall, the betacyanins extraction employing the optimized LBEPS showed the significant highest values of betacyanins concentration in alcohol-rich top phase (C ) and partition coefficient (K) of betacyanins from peel (99.256 ± 0.014% and 133.433 ± 2.566) and flesh (97.189 ± 0.172% and 34.665 ± 2.253) of red-purple pitaya. These results inferred that an optimal betacyanins extraction was successfully achieved by this approach. Also, the LBEPS with the peel and flesh showed phase volume ratio (V ) values of 1.667 and 2.167, respectively, and this indicated that they have a clear biphasic separation. In addition, the peel and flesh extract obtained from the optimized LBEPS demonstrated different variations of red color as well as their antioxidant properties were well-retained. This article introduces a new, reliable, and effective bioseparation approach for the extraction of biomolecules, which is definitely worth to explore further as a bioseparation tool in the downstream bioprocessing.
如今，下游生物加工行业倾向于开发绿色高效的生物分离技术。由于其高染色强度和促进健康的功能特性，甜菜色素目前在食品科学领域受到越来越多的关注。在本研究中，提出了一种新型的绿色集成工艺——液-双相电分配系统（LBEPS），用于从红紫色火龙果的果皮和果肉中提取甜菜色素。首先，将初始设置的LBEPS提取甜菜色素的效果与液-双相分配系统（LBPS）进行比较，结果表明两种系统的甜菜色素提取效果相当。接下来，对LBEPS进行进一步优化以更好地提取甜菜色素。研究了几个操作参数，包括操作时间、施加的电压以及系统中石墨电极的位置。此外，还对优化后的LBEPS与经过电系统优化条件（作为后处理）的LBPS进行了比较，并进行了颜色表征和抗氧化性能评估。总体而言，采用优化后的LBEPS提取甜菜色素，在富含乙醇的上相中甜菜色素浓度（C）以及红紫色火龙果果皮（99.256±0.014%和133.433±2.566）和果肉（97.189±0.172%和34.665±2.2,53）中甜菜色素的分配系数（K）均显示出显著的最高值。这些结果表明，通过这种方法成功实现了甜菜色素的最佳提取。此外，用果皮和果肉的LBEPS的相比（V）值分别为1.667和2.167，这表明它们具有明显的双相分离。此外，从优化后的LBEPS获得的果皮和果肉提取物表现出不同的红色变化，并且其抗氧化性能得到了很好的保留。本文介绍了一种用于提取生物分子的新型、可靠且有效的生物分离方法，作为下游生物加工中的生物分离工具，无疑值得进一步探索。
