Department of Chemical and Environmental Engineering, Faculty of Science and Engineering, University of Nottingham Malaysia, 43500 Semenyih, Selangor, Malaysia.
Department of Chemical Engineering, School of Chemical Engineering, Vellore Institute of Technology, Vellore 632014, India.
Bioresour Technol. 2019 Sep;288:121519. doi: 10.1016/j.biortech.2019.121519. Epub 2019 May 20.
Liquid biphasic flotation (LBF), an integrated process of liquid biphasic system (LBS) and adsorptive bubbles flotation, was used for the purification of C-phycocyanin from S. platensis microalgae. Various experimental parameters such as type of phase forming polymer and salt, concentration of phase forming components, system pH, volume ratio, air flotation time and crude extract concentration were evaluated to maximise the C-phycocyanin recovery yield and purity. The optimal conditions for the LBF system achieving C-phycocyanin purification fold of 3.49 compared to 2.43 from the initial LBF conditions was in polyethylene glycol (PEG) 4000 and potassium phosphate combination, with 250 g/L of polymer and salt concentration each, volume ratio of 1:0.85, system pH of 7.0, air flotation duration of 7 min and phycocyanin crude extract concentration of 0.625 %w/w. The LBF has effectively enhanced the purification of C-phycocyanin in a cost effective and simple processing.
液-液两相浮选（LBF）是液-液双相体系（LBS）和吸附气泡浮选的集成工艺，用于从螺旋藻微藻中纯化 C-藻蓝蛋白。评估了各种实验参数，如相形成聚合物和盐的类型、相形成成分的浓度、体系 pH 值、体积比、空气浮选时间和粗提取物浓度，以最大限度地提高 C-藻蓝蛋白的回收率和纯度。与初始 LBF 条件相比，LBF 系统实现 C-藻蓝蛋白纯化倍数为 3.49 的最佳条件是聚乙二醇（PEG）4000 和磷酸钾组合，聚合物和盐的浓度各为 250g/L，体积比为 1:0.85，体系 pH 值为 7.0，空气浮选时间为 7 分钟，藻蓝蛋白粗提取物浓度为 0.625%w/w。LBF 有效地提高了 C-藻蓝蛋白的纯化效果，成本效益高，处理简单。
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