Kose Yavuz Bulent, Iscan Gokalp, Goger Fatih, Demirci Betul, Elmacı Ceren
Faculty of Pharmacy, Department of Pharmaceutical Botany, Anadolu University, Eskisehir, Turkey.
Faculty of Pharmacy, Department of Pharmacognosy, Anadolu University, Eskisehir, Turkey / Yunus Emre Vocational School, Anadolu University, Eskisehir, Turkey.
Pak J Pharm Sci. 2017 Nov;30(6):2047-2052.
In the present study hydrodistilled essential oil and total methanol extracts of Tanacetum argenteum subsp. flabellifolium have been evaluated for their antimicrobial and antioxidant effects. The chemical composition of the oil and the crude extract were determined by GC/FID, GC/MS and LC/DAD/ESI-MS systems respectively. β-thujone (47.1%), α-pinene (19.1%) and α-thujone (10.5%) were the main compounds of the essential oil while the 5-Ocaffeoylquinic acid, 1,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid were identified as flavonoid content of the crude extract. The oil and the methanol extract were demonstrated moderate antimicrobial effects (MIC range; 0,062-2,0 mg/mL) against 21 different pathogenic micro organism. Total phenolic content was determined as 63 mg GAE in g extract and the DPPH radical scavenging effect was determined as 0.16 mg/mL (IC) and TEAC was determined as 0.21mMol.
在本研究中，对香叶菊绢毛亚种的水蒸馏精油和甲醇总提取物的抗菌和抗氧化作用进行了评估。分别通过气相色谱/氢火焰离子化检测器（GC/FID）、气相色谱/质谱联用仪（GC/MS）和液相色谱/二极管阵列检测器/电喷雾电离质谱联用仪（LC/DAD/ESI-MS）系统测定了精油和粗提取物的化学成分。β-侧柏酮（47.1%）、α-蒎烯（19.1%）和α-侧柏酮（10.5%）是精油的主要成分，而5-咖啡酰奎宁酸、1,5-O-二咖啡酰奎宁酸、4,5-O-二咖啡酰奎宁酸被鉴定为粗提取物中的黄酮类成分。精油和甲醇提取物对21种不同的致病微生物表现出中等抗菌作用（最低抑菌浓度范围为0.062 - 2.0毫克/毫升）。提取物中总酚含量测定为每克提取物63毫克没食子酸当量，二苯基苦味酰基自由基清除效果测定为0.16毫克/毫升（半数抑制浓度），抗氧化能力测定为0.21毫摩尔。
