Shaheen Sidra, Aman Afsheen, Qader Shah Aliul, Siddiqui Nadir Naveed
The Karachi Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan.
Deparment of Biochemistry, University of Karachi, Karachi, Pakistan.
Pak J Pharm Sci. 2017 Nov;30(6):2053-2059.
The industrial utilization of enzymes requires the high yield of enzyme production for the synthesis of polymers by microorganisms. Therefore, it is necessary to optimize different production parameters of levansucrase in order to increase its industrial applications. Zymomonas mobilis KIBGE-IB14 was considered as a promising candidate for the large scale production of levan among wide range of microorganisms. The current investigation is aimed to optimize the production parameters of levansucrase by Z. mobilis KIBGE-IB14 isolated from molasses. The results indicated that bacterial growth as well as enzyme production was greatly influenced by both physical and chemical conditions. It was revealed that high enzyme titers were achieved at 30°C with pH 6.5 after 24 hours of incubation in a modified medium. Moreover, the enzyme exhibited its induction in the presence of sucrose used as a substrate. Thus, the present study demonstrated that newly isolated Z. mobilis KIBGE-IB14 can be used as a plausible producer of levansucrase for industrial applications.
酶的工业利用需要通过微生物合成聚合物来实现酶的高产。因此，有必要优化蔗糖转化酶的不同生产参数，以增加其工业应用。在众多微生物中，运动发酵单胞菌KIBGE-IB14被认为是大规模生产果聚糖的有前景的候选菌株。当前的研究旨在优化从糖蜜中分离出的运动发酵单胞菌KIBGE-IB14生产蔗糖转化酶的参数。结果表明，细菌生长以及酶的产生都受到物理和化学条件的极大影响。结果显示，在改良培养基中于30°C、pH 6.5下孵育24小时后可获得高酶活性。此外，该酶在以蔗糖作为底物时表现出诱导作用。因此，本研究表明新分离的运动发酵单胞菌KIBGE-IB14可作为工业应用中蔗糖转化酶的合理生产菌株。
