Mencher D, Pugatsch T, Wallach M
Kuvin Center for the Study of Infectious and Tropical Diseases, Hebrew University-Hadassah Medical School, Jerusalem, Israel.
Exp Parasitol. 1989 Jan;68(1):40-8. doi: 10.1016/0014-4894(89)90006-4.
RNA was extracted from isolated Eimeria maxima gametocytes and translated in a rabbit reticulocyte cell-free protein synthesis system. The major cell-free translation products from E. maxima gametocyte RNA ranged from 225 to 50 kDa, distinct and different from uninfected chicken intestine cell-free translation products. Rabbit antiserum to E. maxima gametocytes as well as recovered chicken sera specifically precipitated some of the major gametocyte cell-free products. A time course of infected intestine RNA indicated that these cell-free synthesized gametocyte antigens appear at 130 to 138 hr postinfection.
从分离出的巨型艾美耳球虫配子体细胞中提取RNA，并在兔网织红细胞无细胞蛋白质合成系统中进行翻译。巨型艾美耳球虫配子体细胞RNA在无细胞体系中的主要翻译产物分子量范围为225至50 kDa，与未感染鸡肠道的无细胞翻译产物不同且有明显差异。针对巨型艾美耳球虫配子体细胞的兔抗血清以及回收的鸡血清能特异性沉淀一些主要的配子体细胞无细胞产物。感染肠道RNA的时间进程表明，这些无细胞合成的配子体抗原在感染后130至138小时出现。
